SARS-CoV-2 possesses a ~30-kb positive-sense RNA genome encoding ~29 proteins including four structural proteins: spike (S), nucleocapsid (N), matrix (M), and envelope (E)7,8. Homotrimers of the S glycoprotein coat the SARS-CoV-2 virion and engage the viral receptor, angiotensin conver...
To investigate this question, we used a high-throughput mAb isolation platform to generate 459 spike-specific mAbs representing 405 clonally unique Ab lineages from two individuals who were first infected with index SARS-CoV-2 and subsequently vaccinated with a single dose of mRNA-1273 about 5 mon...
To further evaluate the dynamics of the anti-SARS-CoV-2 Ab response, we compared the Ab levels in paired samples from 27 patients. The first sample (TP1) was taken at median day 21 (range 7–64) after the onset of symptoms, and the paired second sample (TP2) was taken at median day...
Interaction of csGRP78 and SARS-CoV-2 spike protein with a dissociation constant of KD = 55.2 nM, describing the affinity between the spike protein ligand and csGRP78 was validated in vitro. Infection rate analyses in ACE2low and GRP78high THP-1 cells - a cell line helpful to study ...
Introduction. Currently, sequencing has been the only tool for the identification of circulating severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants. However, it is known to be an e...
Determining the role of fomites in the transmission of SARS-CoV-2 is essential in the hospital setting and will likely be important outside of medical facilities as governments around the world make plans to ease COVID-19 public health restrictions and attempt to safely reopen economies. Expanding...
SARS-COV-2中和抗体检测试剂盒(sVNT)/SARS-CoV-2 surrogate Virus Neutralization Test Kit可定量检测human的Serum,Plasma样品。 中昊新生是AffinityImmuno在中国的代理商。我们很荣幸为您的研究提供AffinityImmuno产品订购以及帮您解决所有的技术问题。 SARS-CoV-2 surrogate Virus Neutralizing Test (sVNT) (spike prote...
CoV-2 clone. The second variant, EHC-083E (B.1 lineage), containing a D614G mutation within the spike, was the predominant circulating strain at the time of the study and was isolated from a residual nasopharyngeal swab from a patient in Atlanta, Georgia, in March 2020 (SARS-CoV-2/...
Huh-7 cell line (acquired from ATCC, not authenticated), which expresses ACE2 receptor, was infected with pseudovirus expressing the full length of SARS-CoV-2 Spike protein and luciferase reporter gene in the presence and absence of serial dilutions of testing antibodies. Viral entry to the cel...
human serum. In addition, we introduced the SARS-CoV-2 spike-pseudotyped virus into the human serum, and tested it on CE-LIF. The results demonstrated that FAM-nCoV-S1-Apt1 exhibited satisfactory recognition (Fig.1i) and detection linearity (Fig.1j) in the range of 102–105virus particles...