A highly-sensitive sandwich enzyme-linked immunosorbent (ELISA) procedure was developed for detecting human fetuin A (HFA), a specific biomarker for a plethora of various infection and inflammation associated diseases. In this one-step antibody (Ab) immobilization, the capture anti-HFA Ab formed a...
INS Sandwich ELISA 产品说明书 Human INS Sandwich ELISA Kit Datasheet Please read it entirely before use Catalogue Number:KE00045 Size: 96T 96T Sensitivity: 1.3 pmol/L Range: 7.8 - 500 pmol/L Usage:For the quantitative detection of human INS concentrations in serum and plasma.This ...
Build your own ELISA Find protocols below for a standard sandwich ELISA using a 96-well plate for the detection techniques—colorimetric (chromogenic), chemiluminescent, and fluorescent detection. Overall procedure Attachment of capture antibody specific to target protein to a microplate Addi...
Build your own ELISA Find protocols below for a standard sandwich ELISA using a 96-well plate for the detection techniques—colorimetric (chromogenic), chemiluminescent, and fluorescent detection. Overall procedure Attachment of capture antibody specific to t...
This procedure, which cannot be done with the HRP ELISA, increases the accuracy of the antigen detection and assures that the antigen concentration in all samples fits to the standard curve. To validate hADA1 as a detection enzyme, we used both HRP and hADA1-based ELISA to measure the con...
An enzyme-linked immunoassay (ELISA) procedure has been developed to quantitate the amount of elastin produced by cultured porcine aortic smooth muscle cel... M.,Gabriella,Giro,... - 《Collagen & Related Research》 被引量: 59发表: 1984年 A rapid and sensitive cellular enzyme-linked immunoabsor...
The whole assay procedure is controlled and evaluated by a computer. Rabbit IgG and HRPlabelled protein A are also detected by a fluorometer, which is introduced into the flow system. In the flow-injection sandwich ELISA, the slope of the calibration curve is 0.4491 in the range of 0 and ...
We developed an ELISA procedure to measure OxLDL present in mouse circulating plasma, by modification of the previously established method. This method would be a useful tool to study the behavior and function of OxLDL in early development of atherosclerosis. 展开 ...
tissue revealed that the most appropriate procedure was a double-sandwich ELISA using polyclonal antibodies for capture and monoclonal antibodies for detection... GL Lamka,JH Hill,DC Mcgee,... - 《Phytopathology》 被引量: 100发表: 1991年 A COMPARISON OF DIFFERENT METHODS FOR DETERMINING ELASTASE...
We developed a procedure to selectively quantify NLF using sandwich enzyme-linked immunosorbent assay (ELISA), utilizing a polyclonal anti-LF capture antibody paired with a monoclonal anti-nitrotyrosine detector antibody. The assay was applied to quantify NLF in samples of pure LF nitrated via two ...