Sandwich ELISA diagram courtesy ofUniv. of Florida.Click to enlarge. Materials Bicarbonate/carbonate coating buffer (100mM) Dilute the antigen in coating buffer to immobilize them to the wells: 3.03g Na2CO3, 6.0g NaHCO3, 1000ml distilled water, pH 9.6. Blocking solution: Common blocking agents;...
Initial CRP concentrations in saliva were determined using ELISA. CRP was subsequently spiked into saliva samples to reach desired concentrations. As shown in Figure S1, no hook effect was observed at the test line in the range between 1 ng/mL to 10 μg/mL; however, fluctuation in T/C ...
Double antibody sandwich ELISA (DAS-ELISA) and pol Borsha,Rani,Baruah,... - 《Annals of Plant Protection Sciences》 被引量: 2发表: 2016年 Method and apparatus for managing a user's health 1 shows a block diagram of an embodiment of a health management system 100 having a client computer...
ferrocenedimethanol in PBS with the same settings as those used in characterization, except for the DC potential and AC excitation, being 170 mV and 5 mV, respectively. Chronoamperometry was performed with the 1-Step Turbo TMB-ELISA Substrate Solution for 120 s with a fixed potential at 0.0...
To this end, we describe the development and application of an ELISA for quantifying residual dsRNA in non-adjuvanted mRNA vaccine samples ranging from crude IVT reaction products to fully purified bulk drug substance, as well as “lessons-learned” during assay development. We describe the ...
African swine fever virus;double-antigen sandwich ELISA;diagnosis;p30 protein;prokaryotic expression system 2. Materials and Methods 2.1. Serum Samples Positive standard sera for ASFV, pseudorabies virus (PRV), classical swine fever virus (CSFV), and porcine circovirus type 2 (PCV2) were purchased ...
To date, some research efforts have been made to monitor 2-AG, such as high- performance liquid chromatography/mass spectroscopy (HPLC-MS), liquid chromatog- raphy/mass spectroscopy (LC-MS), LC-MS/MS, and enzyme-linked immunosorbent as- say (ELISA) [14–18]. Routine approaches, despite ...
Then, culture supernatants were harvested, and the levels of IL-6, IL-8, IL-12, and TNF-α were measured using specific ELISA kits (Neobioscience, Shenzhen, China), according to the manufacturer's recommendations. Cytokine concentration was read from the standard curves and expressed as pg/mL...