In addition, these four plating media, plus xylose lysine desoxycholate agar (XLD) were evaluated using nonspiked chicken liver and pork sausage samples. The concentration of 1.2 g/L of pp3 in XLT4 gave the best overall results. In virtually all cases, the Salmonella colonies were larger ...
Suspect Salmonella colonies were subjected to biochemical identification by BBL Enterotube II (BD Franklin Lakes, NJ USA) and serological identification using Salmonella group-specific antisera. XLD agar allows a primary distinction of H2S+ and H2S- Salmonella strains. This is a semi-quantitative ...
Of the 150 samples, 11 (7.33%) produced characteristics pink colony with black center on XLD agar medium, and all were culturally and biochemically confirmed to beSalmonella. All possessed serovar-specific geneSpeFand reacted uniformly with group D antisera, suggesting that all of the isolates ...
Some strains ofShigella, such assonneiandS. dysenteriaeserovar 1, may ferment lactose relatively slowly, and colonies change to lactose-fermenting after cultivation for 2 or more days. A few non-pathogenic organisms may grow onSalmonella Shigella References SS Agar. Hardy Diagnostics....
Working cultures of the strains were maintained at 4°C on xylose lysine deoxycholate (XLD) agar (Acumedia-Neogen) plates. All six strains were hydrogen sulfide producers, indicated by the formation of black-centered colonies on the XLD agar. Before each experiment, the Salmonella strains were ...
Samples were enriched, plated on XLD agar and evaluated for Salmonella-typical colonies. In four separate trials, WHR significantly reduced the recovery of SE. No SE was detected in two trials and a greater than 70% reduction was seen in the other two trials. ST was also significantly ...
Then, one loop was streaked on BG & XLD agars, and incubated at 37C for 24 hr. After purified on blood agar, the suspected colonies were confirmed by routine biochemical & serological tests. Out of 85 samples, five (5.8%) were positive for Salmonella. Of these, four isolates were ...
The plates (BGA and XLD) were examined for the presence of char- acteristics Salmonella colonies. A single positive colony showing red color with a black center on XLD and red color on BGA agars were subjected for biochemical tests for confirmation. Data processing and analysis Data were ...
A loopful from selenite F and samples of liver and yolk sac were streaked on XLD and S.S agars. After incubation, the suspected colonies were inoculated into TSI agar for biochemical confirmation. The disk diffusion method on Muller Hinton agar was used to determine the susceptibility to ...
A Salmonella-suspected colony on the BGA plates was expected to be only the nalidixic acid-resistant challenge strain, whereas on the XLD plates, colonies of both the challenge and vaccine strain were able to grow. From these XLD or BGA plates, one Salmonella-suspected colony was inoculated in...