PROTEINPHAGEPM2Bacteriophage PRD1 is a tailless membrane-containing double-stranded (ds) DNA virus infecting a variety of Gram-negative bacteria. In order to affect cell lysis, like most dsDNA phages, PRD1 uses the holin-endolysin system. In this study, we identified two accessory lysis genes,...
Pellet was then resuspended into Nuclei Lysis Solution (Promega) containing 0.05% SDS, 0.025% deoxycholate and 200 µg ml−1 RNase A at 37 °C for 20 min to lyse the cells and Protein Precipitation Solution (Promega) was added. DNA was then precipitated using isopropanol. The ...
To address whether the M2 TAM-secreted PTN functions as a paracrine factor to promote GSC tumour growth, we investigated whether silencing PTN expression in the M2-like TAMs could attenuate the pro-tumorigenic effect of TAMs on GSC-driven tumour growth. Since primed U937 cells possess major macro...
16. Childs LM, Goater JJ, O'Keefe RJ, Schwarz EM., Efficacy of etanercept for wear debrisinduced osteolysis. J Bone Miner Res. 2001 Feb;16(2):338‐47. 17. ElKayam O, Yaron M, Caspi D., From ...
In order to affect cell lysis, like most dsDNA phages, PRD1 uses the holin-endolysin system. In this study, we identified two accessory lysis genes, XXXVI and XXXVII, coding for proteins P36 and P37, respectively. Using genetic complementation assays, we show that protein pair P36/P37 is...
CELL-LYSISNUCLEOTIDE-SEQUENCEGENE-PRODUCTMUTAGENESIStext>Transposon insertions in the Rz gene of bacteriophage lambda block lysis if the medium contains divalent cations at concentrations greater than 5 mM, but otherwise cause no change in phenotype. The Rz protein is thought to have an endopeptidase ...
Molecular cloning, overexpression of Rz lysis gene of phage λ, and subcellular localization of its protein product. In: de Pedro MA, Hoeltje JV, Loeffelhardt W, editors. Bacterial growth and lysis. Plenum Press; New York and London: 1993a. pp. 269–276....
Molecular cloning, overexpression of Rz lysis gene of phage λ, and subcellular localization of its protein product. In: de Pedro MA, Hoeltje JV, Loeffelhardt W, editors. Bacterial growth and lysis. Plenum Press; New York and London: 1993a. pp. 269–276....