Spanins are phage lysis proteins required to disrupt the outer membrane. Phages employ either two-component spanins or unimolecular spanins in this final step of Gram-negative host lysis. Two-component spanins like Rz-Rz1 from phage lambda consist of an integral inner membrane protein: i-spanin...
Molecular cloning, overexpression of Rz lysis gene of phage λ, and subcellular localization of its protein product. In: de Pedro MA, Hoeltje JV, Loeffelhardt W, editors. Bacterial growth and lysis. Plenum Press; New York and London: 1993a. pp. 269–276....
The final step in the phage infection cycle: the Rz and Rz1 lysis proteins link the inner and outer membranes Bacteriophage lambdaViral ProteinsBacteriolysisAmino Acid SequenceModels, BiologicalMolecular Sequence DataBacteriophage λ has four adjacent genes – S , R , ... J Berry,EJ Summer,DK St...
In the lambda phage display system, gpD and pV proteins are commonly used to fuse foreign peptides or proteins. Early studies of lambda phage fusion peptides were performed by fusing peptides or proteins to the C-terminus of the tail protein gpV [156]. The C-terminus of pV is not essent...
Like the plaque-forming experiments, only λ cI antisense phage could lyse the λ cI 857 lysogenic E. coli HL012 (Figure 4C). It has been reported that expression of the Rz gene increases when the lysis of E. coli occurs by λ infection.23 After phage infection, the gene expression ...
Traditionally, the organic extraction method for DNA purification originally developed for bacteriophage Lambda32 is used to produce highly pure DNA samples, which are sufficient even for the rigorous sample quality demands of the newest sequencing technologies. Superior results have bee...
each of which consists of six copies of the tail protein pV [117,135,155]. Lambda phage belongs to temperate phage, but it has the common qualities of lytic phage and temperate phage. It can follow a lytic or lysogenic life cycle [92]. As an effective delivery vehicle, this part discus...
1990. Oligomerization of the bacteriophage lambda S protein in the inner membrane of Escherichia coli. J. Bacteriol. 172, 912–921. CAS PubMed Central PubMed Google Scholar Zhang, N. and Young, R. 1999. Complementation and characterization of the nested Rz and Rz1 reading frames in the gen...
Other phage genes that encode secondary lysis proteins such as Rz/Rz1 were not identified in KS10. Transposition The proteins involved in transposition of KS10, specifically those encoded by gene 16 and gene 17, show the most homology to a transposase and transposition protein found in the ...
lysis geneA plasmid (pTU9) containing the lambda (λ) phage lysis genes S(-)RRz and the biosynthetic genes phbCAB of poly-β-hydroxybutyrate (PHB) was constructed and transformed into E.coli JM109. Cultured in Luria-Bertani (LB) medium with 20 g/L glucose, E.coli JM109 (pTU9) ...