通常无法确认这些错误的原因。所有故障排除流程的第一步是检查实验方案,然后重做实验。检查实验方案(参见附录A,本指南的实验方案),甚至要求同事(最好是经验丰富的分子生物学家)审查实验计划,是相当重要的做法。一位博士后研究员在运行PCR多次失败后,意识到PCR预混试剂缺少dNTPs,这个警示性故事提醒我们,即使是最优秀的科...
[1] Ramesh R, Munshi A, Panda SK. Polymerase chain reaction. Natl Med J India. 1992 May-Jun;5(3):115-9. [2] Lorenz TC. Polymerase chain reaction: basic protocol plus troubleshooting and optimization strategies. J Vis Exp. 2012 May 22;(63):e3998. [3] Ghannam MG, Varacallo M. S...
Find additional tips, troubleshooting help, and resources within ourReal-Time PCR and Digital PCR Applications Support Center. You can use this protocol for both RNA and DNA targets. During thermal cycling, the reverse transcription step will not affect performance with DNA targets....
Tips and troubleshooting Scaling up: moving from research to large-scale RNA production for mRNA therapeutics Why lyo-ready mixes are crucial for qPCR assay development It's a snap! 9 considerations for easy multi-fragment cloning Best practices for RNA-seq: Optimizing sample prep Designing...
PCR,Troubleshooting,Optimization,qPCR,real-time PCR,RT-PCR,quantitative PCR,kinetic PCR,978-1-904455-72-1,9781904455721MO BIO LaboratoriesIncCarlsbadUSA and BitesizeBioUK (respectively)
Refer to theuser manualfor details on reaction setup and troubleshooting tips. Cell Lines Validated for Use with the SingleShot™ SYBR®Green One-Step Kit The SingleShot™ SYBR®Green One-Step Kit has been validated for use with a number of common human and rodent cell lines, such as...
[5]Troubleshooting [6]Relatedproducts CAUTION Allreagentsinthiskitareintendedforresearchpurposes.Donotusefordiagnosisorclinicalpurposes.Pleaseobserve generallaboratoryprecautionandutilizesafetywhileusingthiskit. -ReverTraAce ® isaregisteredtrademarkofToyoboCo.,Ltd.inJapan. ...
[1] Introduction [2] Components [3] Protocol 1. RNA template for reverse transcription 2. Reverse transcription [4] Application data [5] Related protocol 1. DNase I treatment of total RNA [6] Troubleshooting [7] Related products C AUTION All reagents in this ...
Reliance One-Step Multiplex Supermix is a ready-to-use, room-temperature stable, 4x reaction master mix optimized for sensitive amplification of up to 5 targets in a single reaction.
While a reaction volume is not specified, we typically use 20 µL reactions. Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.订购 订单状态查询 订单支持 货号直购 现货供应中心 电子采购 支持 帮助&支持 联系我们 - ...