该方法结合了多重连接探针扩增(multiplex ligation-dependent probe amplification,MLPA)技术的灵敏、特异、高通量和实时PCR方便快捷、实时检测的特点。通过检测临床116例慢性乙肝患者DNA样本对MLP-RT-PCR方法进行了方法性能评价,其中检测出LAM耐药突变41例(35.3%),ADV耐药突变17例(14.7%)及两者共同耐药突变5例(4.3%)。
Introduction: Real‐time multiplex ligation probe amplification (RT‐MLPA) is a new technique allowing for the concomitant amplification of several RNA from various tissues. It uses gene‐specific probes targeting regions supposed to be close together on RNA molecules such as fusion transcripts but ...
MRC-Holland RT-MLPA ® mRNA DETECTION / QUANTIFICATION PROTOCOL MRC-HollandTranscription, Reverse
GENE EXPRESSION PROFILING USING a RTMLPA ASSAY ALLOWS FOR AN ACCURATE CLASSIFICATION OF PERIPHERAL T-CELL LYMPHOMA AND HIGHLIGHTS NOVEL SUBGROUPS WITHIN PTCLS-NOSGENE EXPRESSION PROFILING USING a RTMLPA ASSAY ALLOWS FOR AN ACCURATE CLASSIFICATION OF PERIPHERAL T-CELL LYMPHOMA AND HIGHLIGHTS NOVEL SUBGROU...
(multiplex ligation-dependent probe real-time PCR, MLP-RT-PCR)方法.该方法结合了多重连接探针扩增(multiplex ligation-dependent probe amplification,MLPA)技术的灵敏,特异,高通量和实时PCR方便快捷,实时检测的特点.通过检测临床116例慢性乙肝患者DNA样本对MLP-RT-PCR方法进行了方法性能评价,其中检测出LAM耐药突变41...
Differences in RT-MLPA gene expression levels in progressors and non-progressors.Jayne, S. SutherlandPhilip, C. HillIfedayo, M. AdetifaBouke, C. de JongSimon, DonkorSimone, A. JoostenLizet, OpmeerMarielle, C. HaksTom, H. M. Ottenhoff...
(177)–RT-MLPA as a New Tool for the Molecular Diagnosis of Allograft Rejection in Formalin Paraffin-embedded Endomyocardial BiopsiesIn heart transplantation CAV is the main cause of late cardiac graft loss. Its pathogenesis is unclear. The rational of this study was to investigate explanted ...
Identification of biomark- ers for tuberculosis disease using a novel dual-color RT-MLPA assay. Genes Immun 2012; 13:71-82.Joosten SA, Goeman JJ, Sutherland JS, Opmeer L, de Boer KG, Jacobsen M, Kaufmann SH, Finos L, Magis-Escurra C, Ota MO, Ottenhoff TH, Haks MC. Identification ...
Pei-qiongLi, JunZhang, Jian-huiFan, Yuan-zhiZhang, Hong-yingHou. (2013) Development of noninvasive prenatal diagnosis of trisomy 21 by RT-MLPA with a new set of SNP markers. Archives of Gynecology and Obstetrics /Li PQ, Zhang J, Fan JH, et al.: Development of noninvasive prenatal ...
For SMN1 copy number analysis, 2 RT-PCR assays revealed indeterminant results and all 1020 other samples were concordant for SMN1 copy number. There were 9 discrepancies in SMN2 copy number determination mostly due to a variability in MLPA analysis. Conclusion: Both MLPA and RTPCR assays give ...