Introduction: Real‐time multiplex ligation probe amplification (RT‐MLPA) is a new technique allowing for the concomitant amplification of several RNA from various tissues. It uses gene‐specific probes targeting regions supposed to be close together on RNA molecules such as fusion transcripts but ...
MRC-Holland RT-MLPA ® mRNA DETECTION / QUANTIFICATION PROTOCOL MRC-HollandTranscription, Reverse
该方法结合了多重连接探针扩增(multiplex ligation-dependent probe amplification,MLPA)技术的灵敏、特异、高通量和实时PCR方便快捷、实时检测的特点。通过检测临床116例慢性乙肝患者DNA样本对MLP-RT-PCR方法进行了方法性能评价,其中检测出LAM耐药突变41例(35.3%),ADV耐药突变17例(14.7%)及两者共同耐药突变5例(4.3%)。
等位基因特异性PCR( allele specific PCR,AS-PCR ),是指利用引物与模板之间的碱基错配可以有效地抑制PCR反应,进而达到模板区分(等位基因区分)的目的。 由于PCR过程中引物延伸是3'端开始的,所以3'末端的碱基对引物的延伸来说处于至关重要的位置。如果这个碱基与模板互补,则引物能不间断延伸,PCR可以正常进行,得到特定...
MLPAPurpose: To compare the accuracy of a commercially available MLPA kit with a laboratory developed RT-PCR assay for the detection of SMN1 and SMN2 copy numbers in clinical samples. Methods: We developed and validated a laboratory developed real time PCR based test capable of detecting SMN1 ...
GENE EXPRESSION PROFILING USING a RTMLPA ASSAY ALLOWS FOR AN ACCURATE CLASSIFICATION OF PERIPHERAL T-CELL LYMPHOMA AND HIGHLIGHTS NOVEL SUBGROUPS WITHIN PTCLS-NOSdoi:10.1002/hon.2437_62WileyHematological Oncology
目的构建1种检测基因点突变的新型MLP-RT-PCR技术.方法将多重连接探针扩增(multiplex ligation-dependent probe amplification,MLPA)技术与实时PCR技术相结合,优化杂交和连接反应条件,建立MLP-RT-PCR反应步骤.设计检测HBV耐药相关基因5个突变位点的4类MLPA探针,根据反应特异性筛选出高特异性探针.同时对5个突变位点进行检测...
They were determined by RT-PCR and/or PCR followed by direct sequencing, though only MLL -PTD was determined by MLPA method. Some survival analyses ... N Shiba,K Ohki,M Park,... - 《Blood》 被引量: 0发表: 2013年 Cooperation of activating Ras/rtk signal transduction pathway mutations an...
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