Reverse transcription-quantitative PCR (RT-qPCR) is widely used for monitoring viruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), in wastewater. Various materials, including plasmid DNA, synthetic nucleic acids, PCR amplicons, genomic DNA, and cDNA, are currently used ...
PCR.The coefficients of variance(CVs)were 0.9%and 1.5%for the intra-assay and inter-assay tests respectively,which indicated good reproducibility.It took only 4 h to complete the whole course of reaction including extraction of viral RNA and the real-time RT-PCR.Detected with real-time RT-...
The percent agreement between NASH and RT-PCR ranged from 77.6 to 100 for cvs. included in this study (AC Novachip, Atlantic, Norchip, Red Pontiac, Russet Burbank, Russet Norkotah, Shepody and Superior). ELISA on leaves was almost as good as NASH and RT-PCR on dormant tubers, but ...
The quantification cycle (Cq) values for each candidate reference gene were retrieved from each analyzed sample's real-time PCR amplification curves. Our real-time RT-qPCR gene expression analysis in eggs, miracidia, cercariae, 48-h-schistosomula, adult males, and adult females showed a Cq in...
文档分类: 论文--毕业论文 系统标签: hsaqpcrmir血浆产前检测方法 目录缩略语表...1英文摘要...
RT-PCR–ELISA of various dilutions of CVS-11 strain Rabies virus. Five micro liter of the amplified product was run on 1 % agarose gel and 5 μl was used in the ELISA. Titre (FFD50) of the virus used for RNA extraction, RT-PCR–ELISA OD405nm values and the interpretation are shown...
Allele-specific reverse transcriptase real time RT-PCR (RT-qPCR) represents a quick and cost-effective method for SNP (single nucleotide polymorphism) genotyping and has been successfully applied for SARS-CoV-2 variant screening. In the present study, we developed a panel ...
The reference genes most widely used for qRT-PCR in different species are those encoding 18S rRNA, actin, tubulin, polyubiquitin and GAPDH, which have commonly been employed in traditional methods of gene expression detection, such as northern blot, RNase protection test and conventional semi-...
Methods in Molecular BiologyMallet,F. (2000) Characterization of RNA using Continuous RT-PCR coupled with ELOSA. In Rapley,R. (ed.), The Nucleic Acids Protocol Handdbook. Humana Press, Totowa, USA, pp. 219-228.Mallet F. Characterization of RNA using Continuous RT–PCR coupled with ELOSA...
making it very difficult to accurately differentially diagnose these diseases on site. In this study, a quadruplex one-step reverse-transcription real-time quantitative PCR (RT-qPCR) for the detection of PRCoV, PRRSV, SIV, and PRV was established. The assay showed strong specificity, high sensiti...