Allele-specific reverse transcriptase real time RT-PCR (RT-qPCR) represents a quick and cost-effective method for SNP (single nucleotide polymorphism) genotyping and has been successfully applied for SARS-CoV-2 variant screening. In the present study, we developed a panel ...
The RT-PCR–ELISA OD405 values had a linear correlation with the FFD50 of rabies virus used for RNA extraction and the R 2 value was found to be 0.966. Fig. 1 RT-PCR–ELISA of various dilutions of CVS-11 strain Rabies virus. Five micro liter of the amplified product was run on 1...
A more stringent test of PCR specificity was performed by sequencing the amplification products of each of the 42 candidate reference genes. Always the sequence of the PCR product matched that of the target cDNA, thereby confirming the exquisite PCR specificity of the developed primer pairs. Figure...
cost-effective butimproves the diagnostic efficiency. PCR requires frequent handling of reaction tubes, which increases the risk of contamination and potential false positive in the results. Furthermore, agarose gel electrophoresis to visualize the PCR amplicon does not allow proper quantitative analysis....