Allele-specific reverse transcriptase real time RT-PCR (RT-qPCR) represents a quick and cost-effective method for SNP (single nucleotide polymorphism) genotyping and has been successfully applied for SARS-CoV-2 variant screening. In the present study, we developed a panel ...
To test GT-MMLV activity, a two-step end point RT-PCR was performed with N1 primers, SuperScript IV RT (Thermo Fisher Scientific, #18090010) or GT-MMLV, and GoTaq (Promega. #M3001). The template was 100,000 copies of Quantitative Synthetic SARS-CoV-2 RNA: ORF, E, N (ATCC® VR...
(RT–RAA). This method achieves accurate detection of H3 AIV by designing specific primers and probes without cross-reactivity with other viruses. The results show that the method exhibits high sensitivity and specificity and is highly consistent with the results of real-time quantitative PCR (RT...