猪感染SVV-Ⅰ后出现水疱的临床表现与感染口蹄疫(FMD)、猪水疱病(SVD)、水疱性口炎(VS)等极为相似,主要都是鼻子和冠状带出现水泡、跛行、厌食、嗜睡、发烧,并且持续排毒。 目前,世界上有不少实验室已经研制出一些诊断SVV-1的方法,如病毒分离、病毒中和、竞争Elisa、传统的RT-RCR和SYBR绿色荧光实时定量RT-PCR(rRT-...
In conclusion, the novel Uni Kor-H9 assay enables more rapid and efficient diagnosis than the "traditional" method of virus isolation followed by subtyping RT-PCR. Application of the new H9 rRT-PCR assay to AI active surveillance programs will help to control and manage Korean H9 AIVs more ...
Minimizing errors in RT-PCR detection and quantification of SARS-CoV-2 RNA for wastewater surveillance 2022, Science of the Total Environment Show abstract G-quadruplex based biosensor: A potential tool for SARS-CoV-2 detection 2020, Biosensors and Bioelectronics Show abstract Use of the variplex...
COVID-19;SARS-CoV-2;multiplex RT-PCR;in-house RNA isolation;silica-based RNA isolation;magnetic beads RNA isolation 1. Introduction Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a positive-sense single-stranded RNA virus and is the causative agent for coronavirus disease 2019 ...