What is the source of DNA polymerase in PCR? What is the difference between ligases and DNA polymerase? For what purpose is the polymerase chain reaction (PCR) used? Why is nested PCR useful in diagnostic virology? Why are the housekeeping genes ...
RNA isolation, cDNA library preparation, qRT-PCR and sequencing Totally, the transcription profiling of samples at six time points were evaluated, with two biological replicates at each time point. In the proliferation stage, three samples in different time points were selected: (1) Sample 1, cel...
Difference in apoptosis-associated genes expression profiling and immunohistology analysis between Kashin-Beck disease and primary osteoarthritis细胞凋亡相关基因,大骨节病,骨关节炎,原发性,免疫组化,表达谱,RT-PCR分析,程序性细胞死亡Kashin 山涧疾病(KBD ) 是长期、使充分已知的病原学变形的地方性的骨关节炎,...
(2020) 7:5 https://doi.org/10.1186/s40643-019-0293-1 RESEARCH Open Access Astaxanthin accumulation difference between non‑motile cells and akinetes of Haematococcus pluvialis was affected by pyruvate metabolism Lei Fang, Jingkui Zhang, Zhongnan Fei and Minxi Wan* ...
For quantitative RT-PCR (qRT-PCR), 3 μg of total RNA was used to synthesize cDNA with the PrimeScriptTM Ⅱ cDNA Synthesis Kit (Takara Japan). Three independent biological and three technical replicates of each sample were performed and analyzed. qRT-PCR was performed using the SYBR Premix ...
The preamplified cDNA was input into the RT2 Profiler™ PCR Array for Human NFκB Signaling Pathway (Qiagen), a qRT-PCR array that allows for the simultaneous mRNA profiling of 84 genes related to NFκB signaling, in addition to housekeeping genes. An array for each case was run on an ...
MATERIALS AND METHODS Study Design Aiming to identi y the di erential gene expression between CD and UC, we selected a microarray dataset that has been validated by quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (GEO acces- sion No. GSE16879). 6 A total...
Why is PCR used in the process of DNA sequencing? What is the difference between ligases and DNA polymerase? What is a difference between DNA replication and RNA transcription? How do you determine the initial amount of DNA when using qRT-PCR? ...
qRT-PCR analysis showed the expression levels of core genes related to KEGG pathways significantly †Fuqiang Niu, Zihan Liu and Yongjie Liu contributed equally to this work. *Correspondence: Hui Sun Sunhui628@sina.com Liping Zhang lpzhang8@126.com Xiyue Song songxiyue@nwafu.edu.cn Full list...
The quantitative real-time PCR analysis The conditions of quantitative real-time PCR (qRT- PCR) analysis were based on our previous research [56]. All plant samples were collected in six biological repli- cates and immediately frozen in liquid nitrogen. Total RNA was derived from the frozen ...