Interestingly, this domain is separate from its degradation signals (degrons), suggesting that functional overlap of sequences that activate transcription and signal degradation may not be as common as previously thought. We further demonstrate that the intracellular proteasome activity is correlated with...
One degron leads to ubiquitylation on internal lysine(s), whereas the other is independent of ubiquitylation. Stabilization of RPN4 requires inhibition of internal ubiquitylation and inactivation of the ubiquitin-independent degron. RPN4 represents the first proteasomal substrate in that can be degraded ...
The identification of Lys-187 as the preferential ubiquitination site and NAD as an essential sequence for the Ub-dependent degradation of Rpn4 prompted us to determine whether Lys-187 and NAD (including minimal flanking sequences) constitute a sufficient degron. To this end, we fused residues ...
Furthermore, we confirm the activity of this degron in yeast. Thus, the Rpn4 central domain is an effective interspecies degradation signal.doi:10.1016/j.febslet.2014.08.017A.V. MorozovW.A. Engelhardt Institute of Molecular Biology RAS, Moscow, RussiaD.S. Spasskaya...
One degron leads to ubiquitylation on internal lysine(s), whereas the other is independent of ubiquitylation. Stabilization of RPN4 requires inhibition of internal ubiquitylation and inactivation of the ubiquitin-independent degron. RPN4 represents the first proteasomal substrate in S. cerevisiae that ...