此外,通过优化反应体系(温度、时间、醋酸镁浓度),进一步提高扩增效率,可解决RPA-LFS结果出现假阳性等问题[7]。 尽管RPA-LFA技术操作便捷、时间短,但由于其试剂、耗材基本来源于进口(TwistDx,英国剑桥),检测成本较为高昂,目前仅在实验室...
The limit of detection (LOD) of RPA with gold nanoparticle-based LFA readout is estimated at 1 parasite per reaction, but LOD can be 100-fold better, 0.01 parasites per reaction, for LFA based on SBMSO. This approach may be useful for sensitive and cost-effective poi...
1～7:反应温度分别为20、25、30、35、40、45和50℃;8～14:反应时间分别为0、5、10、15、0、25、30min。 图2为实施例3中rpa-lfa检测日本血吸虫基因组dna的敏感性及特异性评价的试纸条显色结果图。其中,a:rpa-lfd检测梯度浓度日本血吸虫基因组dna结果;b:rpa-lfd检测多种吸虫基因组dna结果。1～7:分别为10...
与普通PCR方法相比,RPA‑LFA具有以下优势:首先,RPA‑LFA属于等温扩增技术,对仪器设备要求低,仅需要恒温水浴锅即可完成反应。其次,RPA‑LFA检测速度快,反应时间在40min,比常规PCR反应时间要短。第三,可实现检测结果的可视化,这些特点使得本发明建立的RPA‑LFA方法可以用于基层单位普通实验室ASFV快速检测和鉴别诊断...
To optimize the accuracy of the assay in the field, we combined RPA-CRISPR/Cas12a-LFA with a visualization and digitization instrument to solve the problem of false-negative results from weakly positive samples on the test strips. First, we designed a crRNA probe for the CRISPR/Cas12a ...
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The amplified RPA-nfoproducts ofA. marginale, A. ovis,andA. phagocytophilumwere detected in lateral flow assay (LFA). The presence of control line C confirmed the lateral flow assay was working properly, test line 1 showed the internal control (GADPH gene), and test line 2 verified the prese...
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30、3)取rpa扩增产物加至用于进行crispr/cas12a的反应体系中,进行荧光检测反应(即rpa/cas12a可视化荧光检测反应或rpa/cas12a荧光检测反应)或者侧流层析试纸条检测(即rpa/cas12a-lfa反应)。 31、进一步的,步骤1)中,提取红火蚁疑似样品的基因组dna的方法是取红火蚁的疑似样品,放在干净的1.5 ml无菌离心管中,在各管...
CD2 (LFA-2) is a monomeric surface antigen (MW range 45-58 kDa) of the human T-lymphocyte lineage that is expressed on all peripheral blood T cells. CD2 is one of the earliest T-cell markers, being present on more than 95% of thymocytes and it is also found on some natural killer...