Using this system, doxycycline treatment of the Gpr30-rtTA;TetO-Cre;R26-TdTomato mice revealed that TdTomato was mainly expressed in GIF-positive chief cells and not in isthmus stem cells [80]. Importantly, using Gpr30-rtTA;TetO-KrasG12D-IRES-TdTomato mice, Hata et al. found that Gpr30...
GzmbCre;Rosa26ACTB-tdTomato, -EGFP/+ mice have a low frequency of GFP+ mature NK cells.Xu, YiyingEvaristo, CésarAlegre, MariaLuisaGurbuxani, SandeepL. Kee, Barbara
The donor DNA template (Fig. 2A) consists of a splice acceptor (SA), a promoterless tdTomato gene, and an inverted EGFP (iEGFP) flanked by homologous arms. To realize Cre-mediated removal of the tdTomato gene, inversion the expression of the iEGFP, and consequently the expression of ...