the obtained ROSA26 promoter is used for expressing GFP of an exogenous gene; and the GFP protein is widely and stably expressed in pigs, so that the promoter can solve the problems of the instability of expression of the transgenic porcine endogenous gene and the unpredictability of position ef...
和Col1a1位点类似,最好在TetO-cDNA-polyA两侧加上隔离子来减少不依赖于四环素的本底表达。 图9. The ApoE TIGRE locus contains an exogenous copy of ApoE cDNA driven by TRE and flanked by four copies of chicken b-globin insulator (ins) sequences, two on each side, and a PGK promoter (P) dri...
In these mice, the ROSA26 promoter/enhancer drives the expression of a Cre-recombinase that is fused to a mutant estrogen ligand-binding domain (ERT2) that prevents Cre from entering the nucleus. This TAM-dependent inducible construct is called “CreERT2.” When TAM is administered, TAM ...
A new transgenic line, R26p-Fucci2, utilizes the Rosa26 promoter and harbors the G(1) and S/G(2)/M phase probes in a single transgene to preserve their co-inheritance. In the other R26R-Fucci2 approach, the two probes are incorporated into Rosa26 locus conditionally. The Cre-mediated...
This Cre-mediated re- arrangement would place EGFP directly under the con- trol of the endogenous pRosa26 promoter (Figure 1E). Another advantage of putting heterotypic loxP sites in the knock-in vector is that virtually any gene of interest could be inserted into the pRosa26 locus by RMCE...
本发明涉及生物技术领域中,用于突变rosa26基因的系统及其应用。 背景技术: 多不饱和脂肪酸(pufas)是一类被广泛研究和关注的脂肪酸。pufas主要包括ω-6和ω-3pufas两种类型,每一种类型都包括多个从短碳链(18c)到长碳链(22c)的、且含有多个不饱和键的脂肪酸。越来越多的研究证明,pufas具有广泛的生物学功能,...
Promoter,GateWay Red,CRISPR shRNA,miRNA 蛋白表达 circRNA,shRNA Cre,Loxp CRISPR,Gateway CRISPR,转座 gRNA Marker miRNA,ncRNA miRNA,shRNA RNA shRNA,ORF shRNA,四环素诱导 tRNA cDNA,GateWay CMV CRISPR,Cas9,Red CRISRP,sgRNA GateWay,ORF lncRNA micRNA ncRNA,miRNA ORF,Cre ORF,Cre,Loxp ORF,CRISPR,Cas...
The mouse ROSA26 locus serves as a port to maintain stable expression of the transgene. Therefore, a bacterial artificial chromosome (BAC) containing the ROSA26 locus was used as a platform to produce proteins by incorporating transgenes with foreign promoters. However, the endogenous promoter of ...
B6.129S7- Gtrosa26 (ROSA26) mice carry a LacZ-neo R insertion on Chromosome (Chr) 6, made by promoter trapping with AB1 129 ES cells. Female C57BL/6J Apc Min /+ (B6 Min /+) mice are very susceptible to the induction of mammary tumors after treatment with ethylnitrosourea (ENU). ...
启动子: CMV promoter 复制子: pUC ori,f1 ori 终止子: SV40 poly(A) signal 质粒分类: 哺乳系列质粒;哺乳荧光质粒;哺乳绿色质粒 质粒大小: 4726bp 质粒标签: C-GFP 原核抗性: 卡那霉素Kan(50μg/ml) 筛选标记: 新霉素Neo/G418 克隆菌株: DH5α等大肠杆菌 ...