结果Rosa26R—YFP小鼠与ER啦cre小鼠的杂交后代共9只,采用基因型分型方法筛选出双阳性转基因小鼠5只;体外培养的c.ki t+细胞部分形态发生变化,提示存在细胞分化;流式细胞术检测到c.ki t+细胞中YFP表达量可达13.7%。结论YFP报告基因系统在O H T诱导的ER眨cre作用下,能有效标记小鼠造血干祖细胞。【关键词】造血...
Quoix N, Cheng-Xue R, Guiot Y, Herrera PL, Henquin JC, Gilon P (2007) The GluCre-ROSA26EYFP mouse: a new model for easy identification of living pancreatic alpha-cells. FEBS Lett 581:4235-4240Quoix, N., Cheng-Xue, R., Guiot, Y., Herrera, P.L., Henquin, J.C., and Gil...
To overcome this limitation, we have developed a new transgenic mouse model, referred to as GluCre-ROSA26EYFP or GYY mice, expressing the enhanced yellow fluorescent protein (EYFP) specifically in α-cells. These mice were obtained by crossing two strains: ROSA26-EYFP [16] and glucagon-Cre ...
We then generated two reporter lines of mice by inserting EYFP or ECFP cDNAs into the ROSA26 locus, preceded by a loxP-flanked stop sequence. These strains were tested by crossing them with transgenic strains expressing Cre in a ubiquitous (尾-actin-Cre) or a cell-specific (Isl1-Cre and ...
Characterization of Cre activity in PLP/CreERT+; Rosa26-EYFPflox/+ mice.doi:10.1371/journal.pone.0089377.g001L. ZhiyongFang JieD. JenniferZhang LingliZuo Jian
sexisletinsulinstreptozotocinhydrocortisonetransdifferentiationThe present study examines differences in metabolic and pancreatic islet adaptative responses following streptozotocin (STZ) and hydrocortisone (HC) administration in male and female transgenic GluCreERT2/Rosa26-eYFP mice. Mice received five daily dos...
We then generated two reporter lines of mice by inserting EYFP or ECFP cDNAs into the ROSA26 locus, preceded by a loxP-flanked stop sequence. These strains were tested by crossing them with transgenic strains expressing Cre in a ubiquitous (尾-actin-Cre) or a cell-specific (Isl1-Cre and ...