国际生物医学工程杂志014年10月第37卷第5期IntJBiomedEngOctober014,V01.37,No.5·论著·E酽一Cre诱导的Rosa6R.YFP报告基因系统标记小鼠造血干祖细胞的条件和效率检测罗琦尹洁孟歆怿杨冰雷蕾孙琰李丹丹王瑾王玺何景华【摘要】目的探索ERBcre诱导的黄色荧光蛋白YFP报告
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Furthermore, lineage tracing of Sox2-CreER; ROSA26-lsl-EYFP mice showed that entire glands in the corpus were fully labeled 15–22 months after tamoxifen administration to activate the Cre recombinase [28]. This observation suggested that Sox2-positive cells are multipotent stem cells that can ...
EYFP is not expressed unless the STOP sequence is excised by a Cre recombinase, which recognizes loxP sites on both sides of the STOP sequence. Glucagon-Cre mice express Cre recombinase specifically in α-cells because expression of the enzyme is under control of the glucagon promoter. Hence, ...
sexisletinsulinstreptozotocinhydrocortisonetransdifferentiationThe present study examines differences in metabolic and pancreatic islet adaptative responses following streptozotocin (STZ) and hydrocortisone (HC) administration in male and female transgenic GluCreERT2/Rosa26-eYFP mice. Mice received five daily dos...
The GluCre-ROSA26EYFP mouse: A new model for easy identification of living pancreatic α-cells The control of glucagon secretion by pancreatic α-cells is poorly understood, largely because of the difficulty to recognize living α-cells. We describe ... N Quoix,CX Rui,Y Guiot,... - 《...
The GluCre-ROSA26EYFP mouse: A new model for easy identification of living pancreatic α-cells The control of glucagon secretion by pancreatic α-cells is poorly understood, largely because of the difficulty to recognize living α-cells. We describe ... N Quoix,CX Rui,Y Guiot,... - 《Fe...
An engineered light-inducible chloride pump,Natronomonas pharaonishalorhodopsin 3 (eNpHR3) enables temporally and spatially precise inhibition of genetically defined cell populations in the intact nervous tissues. In this report, we show the generation of new mouse strains that express eNpHR3-EYFP fusion...
The mouse ROSA26 locus serves as a port to maintain stable expression of the transgene. Therefore, a bacterial artificial chromosome (BAC) containing the ROSA26 locus was used as a platform to produce proteins by incorporating transgenes with foreign promoters. However, the endogenous promoter of ...
(eNpHR3) enables temporally and spatially precise inhibition of genetically def i ned cell populations in the intact nervous tissues. In this report, we show the generation of new mouse strains that express eNpHR3-eYfp fusion proteins after cre- and/or flp-mediated recombination to optically ...