本试剂盒提供了活性氧阳性对照试剂Rosup,以便于活性氧的检测。Rosup是一种活性氧阳性诱导药物,浓度为50mg/mL,根据其荧光信号强度,可分析活性氧的实际水平。 本试剂盒本底低,灵敏度高,线性范围宽,使用方便。 本试剂盒可以测定1000个样品(以96孔板为标准)。 产品组成: 组分 规格 H2DCFH-DA(10mM) 100μl 活性...
活性氧检测试剂盒(Reactive Oxygen Species Assay Kit,也称ROS Assay Kit)是一种利用荧光探针DCFH-DA进行活性氧检测的试剂盒。DCFH-DA本身没有荧光,可以自由穿过细胞膜,进入细胞内后,可以被细胞内的酯酶水解生成DCFH。而DCFH不能通透细胞膜,从而使探针很容易被装载到细胞内。细胞内的活性氧可以氧化无荧光的DCFH生成...
Q: If my cells are methanol sensitive, do I need to increase the volume of the 0.1X diluted dye? A:For cells that are sensitive to methanol, we recommend making further dilutions of the dye to 0.1X or 0.01X final. The volume of the DCFH-DA dilutions used in the assay will not ne...
High ROS levels are widely considered to be a general physiological manifestation of cancer cells, and cancer cells are more likely to reach the toxic threshold of ROS and are more sensitive to redox imbalances than normal cells. Therefore, disrupting the balance of redox states is a promising ...
However, unlike paclitaxel, epothilones were less influenced by P-gp; this may be one of the reasons why epothilones were effective in paclitaxel-sensitive and -resistant tumors25,26. Preclinical studies indicated that epothilones and its analogues had anticancer activity in CRC cells27,28,29. ...
The induction of inflammatory mediators in lung epithelial cells [3–7] or other types of lung cells [8] by CS is mainly regulated by redox-sensitive signaling pathways. CS is a potent oxidant that has the ability to initially cause increases in reactive oxygen species (ROS) in extracellular...
The possible mechanism was the decrease of expression ATP- sensitive potassium (KATP) channels, as evidenced in both diabetic rats and high glucose treated cardiomyocytes [40], which increased the susceptibility to arrhythmia [41]. It is suggested that increased oxidative stress and cardiomyocytes ...
Annexin V, a calcium dependent phospholipid-binding protein with a high affinity for PS, can, therefore, be used as a sensitive probe for the exposure of PS on the cell membrane and hence as a marker of apoptosis. Briefly, double-staining for FITC-Annexin V binding and for cellular DNA...
The oxidant sensitive fluorescent probe DCFH-DA was used to determine intracellular ROS generation. Cells were seeded in a 96-well plate at the density 1×104cells/well and cultured for 24 h, then incubated with DCFH-DA for 30 min at 37°C in the dark. After being washed twice with PBS...
Tetrandrine-induced mitochondrial dysfunction is cyclosporine A-sensitive, suggesting the pres- # These authors contributed equally to this study. * To whom correspondence should be addressed. E-mail lkgong@cdser.simm.ac.cn (Li-kun GONG); jren@cdser.simm.ac.cn (Jin REN) Received 2013-01-...