H2DCFH-DA(10mM) 100μl 活性氧阳性对照(Rosup, 50mg/mL) 1ml 图例:使用活性氧检测试剂盒来显示CHO细胞内活性氧荧光。左图为CHO细胞用试剂盒配备的活性氧阳性对照处理;右图为正常CHO细胞。绿色荧光表明细胞活性氧急剧增加,并能显示其定位。 注意事项: 1、 探针装载后,一定要洗净残余的未进入细胞内的探针,...
Q: If my cells are methanol sensitive, do I need to increase the volume of the 0.1X diluted dye? A:For cells that are sensitive to methanol, we recommend making further dilutions of the dye to 0.1X or 0.01X final. The volume of the DCFH-DA dilutions used in the assay will not ne...
High ROS levels are widely considered to be a general physiological manifestation of cancer cells, and cancer cells are more likely to reach the toxic threshold of ROS and are more sensitive to redox imbalances than normal cells. Therefore, disrupting the balance of redox states is a promising ...
10 µM ROS-sensitive DCFH-DA (Beyotime Biotech, Nantong, China) was utilized to stain the cells in culture medium without FBS for 0.5 hour at 37 °C. Following two washes with PBS, the fluorescence intensity was detected by a FACS Calibur flow cytometer (BD Biosciences, CA, USA) or...
However, unlike paclitaxel, epothilones were less influenced by P-gp; this may be one of the reasons why epothilones were effective in paclitaxel-sensitive and -resistant tumors25,26. Preclinical studies indicated that epothilones and its analogues had anticancer activity in CRC cells27,28,29. ...
The possible mechanism was the decrease of expression ATP- sensitive potassium (KATP) channels, as evidenced in both diabetic rats and high glucose treated cardiomyocytes [40], which increased the susceptibility to arrhythmia [41]. It is suggested that increased oxidative stress and cardiomyocytes ...
The induction of inflammatory mediators in lung epithelial cells [3–7] or other types of lung cells [8] by CS is mainly regulated by redox-sensitive signaling pathways. CS is a potent oxidant that has the ability to initially cause increases in reactive oxygen species (ROS) in extracellular...
The oxidant sensitive fluorescent probe DCFH-DA was used to determine intracellular ROS generation. Cells were seeded in a 96-well plate at the density 1×104cells/well and cultured for 24 h, then incubated with DCFH-DA for 30 min at 37°C in the dark. After being washed twice with PBS...
Tetrandrine-induced mitochondrial dysfunction is cyclosporine A-sensitive, suggesting the pres- # These authors contributed equally to this study. * To whom correspondence should be addressed. E-mail lkgong@cdser.simm.ac.cn (Li-kun GONG); jren@cdser.simm.ac.cn (Jin REN) Received 2013-01-...
The redox-sensitive dye DCFH-DA (Beyotime Biotech, Haimen, China) was used to evaluate the levels of intracellular ROS. Briefly, the cells were incubated with 1% Triton X-100 after being stained with 20 μmol/L DCFH-DA in the dark for 30 min. Then, the fluores- cence was measured at...