aA diagram illustrating the design of drought treatment experiments in sterilized and non-sterilized natural soil.bPhenotypes and (c) quantifications of root hair densities of the 5-day-oldrsl2 rsl4, Col-0, andgl2(two-sided Student’sttest). Data represent mean (bar) ± standard error ...
The auxin transport assay with 3H-labeled indoleacetic acid (3H-IAA) showed efficient shootward auxin transport from the root tip of P. taeda that was sensitive to the N-1-naphthylphthalamic acid (NPA), an established inhibitor of auxin transport44 (Supplementary Fig. 10d, e). The shootward...
No somatotopic organization is observed in the thoracic DRGs of the rat, but occasional clustering of labeled cells belonging to the same peripheral nerve was noted (Ygge, 1984). DRG neurons in the rat are arranged in pairs or, less frequently, in groups of three enclosed within a common ...
(A) Ribbon diagram of AteIF4E1 in complex with the m7GDP analog (modeled in PyMOL). m7GDP is located in the cap-binding pocket. The Thr140 residue is labeled. (B) The structure of AteIF4E1 is colored according to the electrostatic potential, with the location of Thr140 being highlighte...
After pre-treatment for 10 min with 1 mg mL−1 lysozyme and a dehydration series with ethanol at increasing concentrations (50%, 70%, and 96%, 3 min each), an equimolar mixture of the Cy3-labeled probes EUB338, EUB338II and EUB338III was applied for the detection ...
The single-cell transcriptome atlas enabled us to identify most of the major cell types of the rice radicle (Supplementary Note 2). Clusters 1, 4 and 9 belonged to the epidermis/root hair population (Fig. 1a, see below). These clusters were separated from other cell clusters on the UMAP ...
Metabolic labeling was used to study the differentially phosphorylated membrane proteins upon elicitor or cadmium treatment in cell culture (6, 14). The use of hydroponic growth systems has also made it possible to apply 15N stable isotope labeling using 15N-labeled inorganic salts to intact plants...
The GFP-labeledFocTR4 was provided by College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China. After seven-day’ incubation at 28 °C in dark on PDA medium, freshFocTR4 was washed out by using sterilized water. After filtering by using sterilized six-layer ch...
In addition, hormones-, sugar-, cell cycle-, root development- and biological enzyme-related genes (Tables S4–S8) that were also selected from the heavily enriched KEGG pathways. A statistical analysis of the differentially expressed enrichment pathway diagram is presented in Figure S2. In ...