(2010) A role for codon order in translation dynamics. Cell, 141, 355-367.Cannarozzi, G., N. N. Schraudolph, et al. (2010). "A role for codon order in translation dynamics." Cell 141(2): 355-367.Cannarozzi, G.; Schraudolph, N.N.; Faty, M.; von Rohr, P.; Friberg, M...
An elegant study engineered ahisoperon leader peptide gene reporter inE. colito investigate the local effects of codon context on in vivo translation speed18. Results demonstrated that the rate at which ribosomes translate individual synonymous codons varies considerably, and that the apparent speed at...
The role of silent position in the codon on the protein structure is an interesting and yet unclear problem. In this paper, 563 Homo sapiens genes and 417 ... Wanjun,Gu,and,... - 《Biosystems》 被引量: 214发表: 2004年 In vivo evaluation of the context sequence of the translation init...
resonance energy transfer (smFRET) as a primary tool for investigating how the dynamics of IF1 and IF3 regulate the accuracy with which the translational machinery selects an initiator transfer RNA (tRNA) and the correct messenger RNA (mRNA) start codon during the initiation stage of protein ...
Mouse latexin cDNA (669 nucleotides) was amplified by using the forward primer 5′-CACCATGGAAATCCCACCCACCCAC-3′ and reverse primer 5′-TCACTCCGCCTGCCCTTCC-3′ (start codon for translation is shown in bold) by using Eppendorf Triple Master blunt-ended proof-reading polymerase. The 50 μl ...
The Δ36/347 mutation is a deletion of the segment +36 to +347, where the translation start codon begins at +355. This mutation was made with and without the 3′UTR (see Results and Experimental Procedures). The grk 5′ intron, which follows residue +164 (see Experimental Procedures), ...
This pairing, as well as factors such as the location of aSD binding relative to the start codon and the sequence of the aSD motif can heavily influence translation efficiency. The objective of this thesis is to understand the SD-aSD interactions and how changes in aSD sequences can affect ...
To initiate protein translation, a ribosome binds and assembles an initiation complex in the area of the gene start codon1. When monocistronic mRNA encoding a single gene is translated, spatial considerations that could interfere with ribosome binding are largely irrelevant. However, in bacteria, ...
recruiting the 40S ribosome to the mRNA [4]; ii) the upstream open reading frame (or uORF), a start codon upstream of the primary ORF with an in-frame stop codon that can lie at either the 5′ or 3′ end of the primary start codon [5]; and iii) N6-methyl adenosine (m6A) [6...
(rltgrMO1) was designed to target the region upstream of start codon and that the mRNA used in the rescue experiment contained ORF ofrltgrand not the morpholino target site, in order to ensure that there was no titration effect leading to false positive rescue results. It should also be ...