Mouse myeloma cells have previously been shown (L. B. Schwartz, V. E. F. Sklar, J. A. Jaehning, R. Weinmann & R. G. Roeder, submitted for publication) to contain two chromatographically distinct forms of RNA polymerase III (designated IIIA and IIIB). The enzymes are unaffected by...
Nuclear speckles are proposed to have roles in promoting mRNA maturation and are localized with sites of active RNA pol II transcription. mRNA production and maturation, including transcription, splicing, polyadenylation, and mRNA export, are often coupled and the proteins Amyloid (A) bodies Recently...
The transcription of the mymA operon under acidic stress has been earlier shown to be regulated by VirS, which itself is regulated by acidic pH (12). Studies demonstrated that the virS mutant of M. tuberculosis exhibited altered cell-wall structure, altered mycolic acid content, defective intra...
Human RNase P has non-canonical roles in regulating transcription of small ncRNA genes by RNA polymerase III (Pol III) and rRNA genes by RNA polymerase I (Pol I)37,48,49. Catalytic forms of this RNP exist in proficient initiation complexes assembled on target gene loci50. Human RNase P bi...
Increasing evidence suggests that RNA modification pathways are also misregulated in human cancers and may be ideal targets of cancer therapy. In this Review we highlight the RNA epitranscriptomic pathways implicated in cancer, describing their biological functions and their connections to the disease. ...
1. 1. The role of transcription in the initiation of DNA synthesis and elongation of the DNA chain in Escherichia coli and the bacteriophage ΦX174 has been studied. 2. 2. Concomitant transcription does not appear to be required for semiconservative DNA replication in bacteria, or for conversio...
During HIV reverse transcription, (+) strand DNA synthesis is primed by an RNase H-resistant sequence, the polypurine tract, and continues as far as a 18-nt double-stranded RNA region corresponding to the 3' end of tRNALys,3 hybridized to the viral primer binding site (PBS). Before (+)...
MicroRNA 200c targets genes critical in cellular transition, inflammation, angiogenesis, cell cycle regulation, and matrix remodeling, such as inhibitor of nuclear factor kappa B kinase subunit β (IKBKB), zinc finger E-Box binding homeobox 1 (ZEB1), zinc finger E-Box binding homeobox 2 (ZEB2...
with transcription factors, translating functional peptides from small open reading frames, mediating posttranscriptional regulation via interaction with diverse RNA-binding proteins (RBPs), and acting as miRNA sponges or decoys. In HCC, for example,lncRNA-TUG1promotes cell growth by interacting with ...
Besides the direct influence over mRNA transcription, the circRNA can also control the target's expression via sponging miRNAs or the RNA-binding proteins. Studies have demonstrated the dysregulation of the circRNA-miRNA-mRNA interaction network in the pathogenesis of many diseases, including diabetes....