DNA primaseThe temperature-sensitive yeast DNA primase mutant pri1-M4 fails to execute an early step of DNA replication and exhibits a dominant, allele-specific sensitivity to DNA-damaging agents. pri1-M4 is defective in slowing down the rate of S phase progression and partially delaying the ...
RNases are a broad class of enzymes that are specific for cleaving RNA molecules. RNases are commonly used in molecular biology laboratories during the purification of DNA and proteins. Different sub-classes of RNase are present, such as RNase A, RNase H, RNase P, RNase L, RNase III, ...
For each of the following describe its role in DNA replication of bacteria: a) Origin of replication. b) DNA B (a helicase). c) Single-stranded binding protein (SSB). d) Primase. e) DNA polymerase III. f) Tus protein. Identify the enzymes involved in DNA re...
These results suggest that arresting cells in S phase using ts alleles of Pol alpha-primase, prior to inducing the DSB, sequesters some other component that is required for repair. We conclude that DNA synthesis during gene conversion is different from S-phase replication, involving only leading-...
DNA Enzymes DNA Polymerase DNA Primase DNA Replication DNA Synthesis siRNA Introduction In eukaryotes, DNA polymerases (Pols)3 α, δ, and ϵ jointly support DNA replication, and their overall roles in this process have been defined (1). The initiation of DNA replication is catalyzed by the ...
RecQ proteins function in a number of diverse cellular contexts, including replication fork maintenance, DNA damage checkpoint signaling, telomere stability, and recombination regulation (1, 2, 3, 4); as such, RecQ helicases link together several DNA metabolic activities, and the mechanisms that ...
DNA Replication Repair Recombination and Chromosome Dynamics: An iron-sulfur cluster in the C-terminal domain of the p58 subunit of human DNA primase of the p58 subunit. In the crystal structure of the S. solfataricus primase core, the p48 subunit assembles with the N-terminal half of p58...
Recruitment of Polymerase to Herpes Simplex Virus Type 1 Replication Foci in Cells Expressing Mutant Primase (UL52) Proteins The ordered assembly of the herpes simplex virus (HSV) type 1 replication apparatus leading to replication compartments likely involves the initial assembl... SD Carrington-Lawr...
Comparison to PDB enabled annotation of three families with no PFAM and KEGG annotations as having functions related to the DNA replication machinery (Additional file1: Table S4). We were interested to find that this ubiquitous function is performed by specific protein families in Crenarchaeota, poss...
What is the role of topoisomerase in DNA replication? What is the role of DNA methylation in regulating transposons (Transposable Elements, TEs; "jumping genes")? What role does DNA play in phenotypic expression in organisms? What is the role of primase in DNA synthesis? What is the ...