Analytical procedures To estimate cell concentration, cells were resuspended in 65 mM phosphate buffer pH 7.5 and absorbance was measured at 600 nm (Pharmacia Biotech Novaspec II Spectrophotometer, Uppsala, Sweden). A600 values and dry cell weight were correlated for each strain. ...
3.1. PDP and EIS measurements of the complex surface layer The influence of phosphate and calcium species, as well as of BSA protein and H2O2, on the resistivity of the oxide layer was investigated, and the BSA protein adsorption and conformational changes during interaction with the oxide layer...
VENOUS blood (1 vol.) diluted with 0.05 M phosphate buffer,pH 7.4 (9 vol.), and clotted with bovine thrombin (Parke, Davis and Co.; 1 unit per ml.) will afterwards dissolve in 2–8 h at 37° C if the diluted blood is handled in siliconized glassware and kept at 0° C for 3...
Hemotoxic concentrations of PNOH were found to be highly unstable in the presence of red cells, though relatively stable in the buffer vehicle alone. Inhibitors of acetylation ( p -aminobenzoic acid [PABA]) and deacetylation (bis-[ p -nitrophenyl]phosphate [BNPP]), used to alter the cyclic ...
Immunoblotting Cells were removed from the plates by scraping, washed twice with phosphate-buffered solution (PBS), and suspended in lysis buffer (150 mM NaCl, 10 mM Tris, 0.2% Triton X-100, 0.3% NP-40, 0.2 mM Na3VO4, and protease inhibitor mixture [Roche Diagnostics Mennheim, Germany]...
EGCg (0.22 mmol/l) in neutral solution (0.1 mol/l phosphate buffer pH 7.0: PBS) quantitatively generated 0.2 mmol/l hydrogen peroxide after 60 min incubation. The bactericidal effect of EGCg is dependent on hydrogen peroxide levels produced by EGCg; moreover, EGCg action was inhibited by ...
Fixed cultures were then cryopreserved in 20% sucrose in 0.1 M phosphate buffer at 4°C and washed before embedding and freezing in optimal cutting temperature compound (Tissue-tek 4538; Miles Laboratories Inc, Naperville, IL, USA). Frozen sections (6 μm) were cut for immunohistochemistry ...
For cancer induction, the cells were resuspended in phosphate buffer (104 cells/ml), and then 50 μl of the cell suspension or vehicle (phosphate buffer) were injected into the right caudal mammary gland of the mouse. The tumor 4T1 cells were kindly provided by Dr. Adriana Bonomo (...
Whole cell genomic DNA was isolated from PBMCs of each subject using the QiAmp DNA Mini Kit (Qiagen, Hilden, Germany). Self-DNA was incubated with a recombinant antimicrobial peptide, LL37 (AnaSpec, CA, USA), at different ratios in 1X phosphate buffer saline (PBS) (pH 7.4) at room te...
Rod outer segments (ROS) were prepared from frozen bovine retinas50. Briefly, under dark conditions (red light) a 45% sucrose solution of phosphate buffer (67 mM K2HPO4/KH2PO4(pH 7.0), 1 mM Mg(CH3COO)2 × 4H2O, 100 µM EDTA-K2, 1 mM DTT, 100 µM PMSF, ...