Phosphate buffer system function in cellular physiology and its consequences in disease states are thoroughly examined in this review. The review defines, explains the concepts, and types of buffers, as well as the variables affecting buffer capacity, starting with an introduction to the importance ...
Phosphate buffer. Anhydrous dipotassium hydrogen phosphate, 215 g, anhydrous potassium dihydrogen phosphate, 169 g, sodium dithionite, 5 g, saponin, 1 g, water to 1 litre. Note: Dissolve the K2HPO4 in water before adding the KH2PO4, then add the dithionite and finally the saponin. This so...
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The principle of calcium phosphate coprecipitation involves incubating DNA and calcium chloride in a phosphate buffer to form DNA-calcium-phosphate precipitates, which are subsequently adsorbed on the cell surface and internalized. From:Comprehensive Biotechnology (Third Edition),2019 ...
of 7.4, Pi exists as both H2PO4(−)and HPO4(2−)and acts as an extracellular fluid (ECF) buffer. Pi is the form transported across tissue compartments and cells. Measurement of Pi in biological fluids is based on its reaction with ammonium molybdate which does not measure organic ...
For ΔPr-SSG blots, cells were lysed in a non- reducing buffer containing 2% NP-40, 50 mM Tris, pH 7.5, 150 mM NaCl, 1 mM benzamidine, 2 mM EGTA, 10 μg/ml aprotinin, 10 μg/ml leupeptin, 1 μg/ml pepstatin, 20 mM ethylmaleimide, and 1 mM PMSF. Western blotting For western...
DMEM (Dulbecco’s modified Eagle medium) supplemented with L-glutamine, FBS (fetal bovine serum), Pen/Strep (10.000 µg/mL, 10.000 units/mL) and phosphate buffer solution (PBS) was purchased from Thermo Fisher company. The Nunc Lab-Tek Chamber Slide system was purchased from Sigma-...
(i.e., no calcium, sulfate or dissolved inorganic carbon) compared to that of standard seawater, and also between various studies where media composition differs. Our experimental pH measurements were calibrated against NIST (NBS) buffer standards, and so experimental measurements are reported on ...
(b) a gene, which hybridizes to a polynucleotide consisting of a nucleotide sequence complementary to the nucleotide sequence set forth in SEQ ID NO: 1 or the nucleotide sequence of the open reading frame thereof under stringent conditions, and which encodes a protein having the function to act...