目的:探讨miRNA-133b对骨髓间充质干细胞(bone marrow derived mesenchymal stem cells,BMSCs)增殖及凋亡的影响,分析miRNA-133b影响BMSCs增殖的可能机制.方法:体外全骨髓贴壁法原代培养大鼠BMSCs,取 生长良好的第2代细胞作为本实验的研究对象.利用siPORT NeoFX转染rno-miRNA-133b模拟物(mimic),抑制剂(inhibitor)...
rno—miRNA-133b对大鼠骨髓间充质干细胞增殖的影响
Objective: To investigate the effect of miRNA-133b on proliferation and apoptosis of bone marrow derived mesenchymal stem cells(BMSCs),and preliminary analyze its possible target and mechanism.Methods: Primary cultured BMSCs of rats were transfected with rno-miRNA-133b mimic and inhibitor by siPORT...
中医药mrna mirna大鼠23samples rno 532mirbase前体总读取计数9690r.pdf,miRBase precursor : rno-mir-532 Total read count : 9690 rno-miR-532-5p read count : 9505 c a a rno-miR-532-3p read count : 185 c u u u g u u remaining reads : 0 a a g a a u c u g g u u g
mouse and rat miRNAs in the current miRbase. Compared to standard miRNA mimics and inhibitors, agomir and antagomir are more resistant to degradation, thus having a lasting effect: minimum for 1 week, up to 5-6 weeks. These products can be used in in vitro or in vivo miRNA functional st...
并探讨茅岩莓多糖对rno-miRNA-484/rno-miR-138-5p/CXCL1信号轴的干预作用,进一步揭示其潜在机制.结果:基于GEO数据库进行筛选,共获得差异表达的miRNA 29个(P<0.05,log2FC<-1或log2 FC>1);MiRWalk数据库预测与CXCL1存在调控联系的miRNA 251个.两者重合13个miRNA,其中上调的有6个,下调的有7个.大鼠肾组织RNA...
目的:探讨miRNA-133b对骨髓间充质干细胞(bone marrow derived mesenchymal stem cells,BMSCs)增殖及凋亡的影响,分析miRNA-133b影响BMSCs增殖的可能机制.方法:体外全骨髓贴壁法原代培养大鼠BMSCs,取生长良好的第2代细胞作为本实验的研究对象.利用siPORT NeoFX转染rno-miRNA-133b模拟物(mimic),抑制剂(inhibitor)及其...
并探讨茅岩莓多糖对rno-miRNA-484/rno-miR-138-5p/CXCL1信号轴的干预作用,进一步揭示其潜在机制.结果:基于GEO数据库进行筛选,共获得差异表达的miRNA 29个(P<0.05,log2FC<-1或log2 FC>1);MiRWalk数据库预测与CXCL1存在调控联系的miRNA 251个.两者重合13个miRNA,其中上调的有6个,下调的有7个.大鼠肾组织RNA...
Osteogenic differentiation, matrix deposition, and mineralization were all significantly increased by rno-miRNA-26a-5p. In addition, this enhancement was not significantly altered by lyophilization and storage. Overall, these findings support the concept of MSN-CC-PEI as a delivery system for gene ...