Moreover, the RNF31 inhibitor gliotoxin inhibited the malignant behavior of HCC cells. Mechanistically, RBCK1 interacted with RNF31 and repressed its ubiquitination and proteasomal degradation. In summary, the present study revealed an oncogenic role and regulatory relationship between RBCK1 and RNF31 ...
Western blot analysis All protein was extracted in radioimmunoprecipitation assay (RIPA) buffer (Solarbio Life Science, Beijing, China) mixed with protease inhibitor on ice-cold plates. Then, the protein concentration was determined with a bicinchoninic acid (BCA) protein assay kit purchased from Sola...
Alternate Names EC 6.3.2.-; FLJ10111; FLJ23501; HOIP; MGC19975; ring finger protein 31; RNF31; ZIBRA; ZIBRAHOIL-1-interacting protein; Zinc in-between-RING-finger ubiquitin-associated domain protein Product Datasheets Product Datasheet
In the presence of the proteasome inhibitor MG132, ERa was stabilized also in the absence of RNF31 and overexpression of RNF31 did not further increase the stability of ERa (Figure 4e). In summary, these data suggest that RNF31 increases ERa protein stability via inhibition of its proteasome...
Treatment with HOIPIN-8, a specific LUBAC inhibitor that suppresses RNF31 ubiquitin ligase (E3) activity, showed similar effects, resulting in decreased cell proliferation and invasion. Our clinical and in vitro data showed that RNF31 is a prognostic factor for HCC that promotes tumor ...