Regulation of HPV16 E6 and MCL1 by SF3B1 inhibitor in head and neck cancer cells. Scientific reports 2014; 4: 6098.Gao, Y., Trivedi, S., Ferris, R. L. & Koide, K. Regulation of HPV16 E6 and MCL1 by SF3B1 inhibitor in head and neck cancer cells. Sci Rep 4, 6098, https://doi...
To visualise the splicing modulation induced by the potential SF3B1 inhibitors on RON, we performed an end-point PCR assay followed by agarose gel electrophoresis. The SUIT-2 cells were seeded in 6-well flat bottom plates and incubated for 24 h with 20 µM of the two most promising compou...
SF3B1MUTerythroblasts demonstrate a significant loss of R-loops. These cells endure a DNA replication stress consisting in accelerated fork progression and single-stranded (ss)DNA exposure, and correlating with increased erythroid cell proliferation and impaired differentiation. The ability of low doses ...
After 24 h, cells were harvested and lysed in a IP buffer (50 mm Tris, pH 8.0, 75 mm NaCl, 5% glycerol, 10 mm CaCl2, cOmpleteTM EDTA-free protease inhibitor (Sigma), 0.5 mm DTT) + 0.5% Triton X-100. Lysates were clarified by centrifugation and a fraction of each sample was set...
Regulation of HPV16 E6 and MCL1 by SF3B1 inhibitor in head and neck cancer cells. Scientific reports. 2014; 4:6098.Gao Y, Trivedi S, Ferris RL, Koide K. Regulation of HPV16 E6 and MCL1 by SF3B1 inhibitor in head and neck cancer cells. Scientific reports 2014; 4: 6098....
To identify potential tumor suppressor genes that are aberrantly spliced and downregulated by mutant SF3B1, we performed RNA-sequencing analysis of SF3B1-mutated primary CLL cells in the presence or absence of cycloheximide (CHX), a translation inhibitor known to inhibit NMD. Our analysis identified...
SF3B1 knockdown experiments in K562 cells resulted in down-regulation of U2-type intron-splicing by RT-PCR. RNA-sequencing analysis of SF3B1 mutants showed differentially used genes relevant in MDS pathogenesis, such as ASXL1, CBL, EZH, and RUNX families. A SF3B pharmacologic inhibitor, mea...