简介: RNA酶保护试验(RNase Protection Assay,RPA)是通过液相杂交的方式,用反义RNA探针与样品杂交,以检测RNA表达的技术。 1. 原理:双链RNA(杂交的)能够抵抗RNA酶的降解。 2. 应用:检测RNA表达 3. 与Northern杂交和RT-PCR比较,RPA有以下几个优点: (1) 检测灵敏度比Northern杂交高。由于Northern杂交步骤中转膜和...
RNase protectionalternative splicingalternative promotersThe RNase protection assay is a highly sensitive technique developed to detect and measure the abundance of specific mRNAs in samples of total cellular RNA. The assay utilizes in vitro transcribed 32 P-labeled antisense RNA probes that are ...
pH to 6.7 and bring up to 100 ml with DEPC treated Q, autoclave 1X Hybridization Mixture 8 ml formamide 1 ml DEPC treated Q 1 ml 10X Hybridization Buffer 2X RNase Buffer 20 mM Tris 7.5 2 ml 1M Tris 7.5 10 mM EDTA 2 ml 500 mM EDTA 8.0 0.6 M NaCl 12 ml 5 M NaCl up to 100...
The RNase Protection Assay (RPA) is a widely used technique for the detection and quantitation of mRNA. The method involves: ‹General Articles Getting Rid of Residual Full Length Probe in Ribonuclease Protection Assays Linearity of RNase Protection Assays Opt...
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The RNaseAlert QC System, when used in conjunction with a fluorometer capable of kinetic analysis, provides a rapid and ultra-sensitive assay for determining RNase A specific activity. The RNaseAlert QC System also detects a variety of other nucleases, including RNase A, ...
METHODS: A Companion to Methods in RNase Protection AssayMa, Ying JunDissen, Gregory ARage, FlorenceOjeda, Sergio R
英文名称:Elution Buffer for RNase Protection Assay 运输:4-25℃ 保存:4-25℃ 有效期:1 Year 货期:2-4 days 其他: 产品介绍:收起 Order Online在线订购 订货信息 产品名称 * 申请类型 * 套数 * 推荐人 真实姓名 * 邮寄地址 * 所属单位 电子邮箱 * 联系电话 * 联系QQ 留言 本公司销售人员将尽快...
RNase ONE: Advantages for nuclease protect ion assay. Promega Notes, 1992, 38: 1~ 7RNase ONE: Advantages for nuclease protection assay. Brewer A B,Murray G,Stateben M. Promega Notes Magazine . 1992Brewer, G., RNase ONE: Advantages for nuclease protection assays, Promega Note, 38 (1992) ...
1) RNase protection assay 核糖核酸酶保护法 2) Ribose 核糖 1. Some evidences for ribose as the reactive flavor precursor of dry-cured meat products; 核糖作为干腌肉制品中具有反应活性的风味前体物的几点证据 2. As an adenine nucleotide precursor, ribose is the primary material used by the heart ...