RNA酶保护分析法(RNase protection assay,RPA) 相关知识点: 试题来源: 解析 此法的原理与核酸酶S1保护分析法基本相同,只是所采用的探针为单链RNA探针,杂交后形成RNA/RNA双链。RNA酶A和RNA酶T1专一性降解单链RNA,双链RNA则不被降解。此法的灵敏度较之核酸酶S1保护分析法还要高出数倍。此法可用于RNA定量、RNA...
简介: RNA酶保护试验(RNase Protection Assay,RPA)是通过液相杂交的方式,用反义RNA探针与样品杂交,以检测RNA表达的技术。 1. 原理:双链RNA(杂交的)能够抵抗RNA酶的降解。 2. 应用:检测RNA表达 3. 与Northern杂交和RT-PCR比较,RPA有以下几个优点: (1) 检测灵敏度比Northern杂交高。由于Northern杂交步骤中转膜和...
pH to 6.7 and bring up to 100 ml with DEPC treated Q, autoclave 1X Hybridization Mixture 8 ml formamide 1 ml DEPC treated Q 1 ml 10X Hybridization Buffer 2X RNase Buffer 20 mM Tris 7.5 2 ml 1M Tris 7.5 10 mM EDTA 2 ml 500 mM EDTA 8.0 0.6 M NaCl 12 ml 5 M NaCl up to 100...
An RNAse protection assay has been established which allows simultaneous analyses of the mRNAs for ALK-2, -3, and -6. As a test system, cultures of pluripotent C3H10T1/2 cell line were incubated in the presence or absence of BMP-7, and the relative levels of the receptor mRNAs were ...
The RNase protection assay is a highly sensitive technique developed to detect and measure the abundance of specific mRNAs in samples of total cellular RNA. The assay utilizesin vitrotranscribed32P-labeled antisense RNA probes that are hybridized in solution to their complementary cellular mRNAs. This...
The RNase Protection Assay (RPA) is a widely used technique for the detection and quantitation of mRNA. The method involves: ‹General Articles Getting Rid of Residual Full Length Probe in Ribonuclease Protection Assays Linearity of RNase Protection Assays Optimal...
The RNase protection assay is a highly sensitive technique developed to detect and measure the abundance of specific mRNAs in samples of total cellular RNA. The assay utilizes in vitro transcribed 32 P-labeled antisense RNA probes that are hybridized in solution to their complementary cellular mRNAs...
The RNase Protection Assay (RPA) is a widely used technique for the detection and quantitation of mRNA. The method involves: ‹General Articles Getting Rid of Residual Full Length Probe in Ribonuclease Protection Assays Linearity of RNase Protection Assays Opt...
RNA酶保护分析法（RNase protection assay）是近年来发展起来的一种检测RNA的杂交技术。其基本原理是利用单链RNA探针，与待测的RNA样品进行杂交形成RNA：RNA双链分子，由于RNA酶可专一性地降解未杂交的单链RNA，而双链受到保护不被降解，经凝胶电泳可以确定目的RNA的长度。该方法灵敏度较Northern杂交法更高，并可进行...