尽管亚基A与原核RNase H2紧密同源,但B和C亚基在原核生物中没有明显的同源性,即使在真核生物中在序列水平上也保守性很差。B亚基介导RNaseH2复合物和PCNA之间的蛋白质-蛋白质相互作用,其将RNase H2定位于复制病灶。 原核和真核RNase H2酶都能切割一条链中的单个核糖核苷酸。然而,它们的切割模式和底物偏好略有不...
\\{RER\\} is most efficient when the ribonucleotide is incised by \\{RNase\\} H2, and further excised by the flap endonuclease \\{FEN1\\} with strand displacement synthesis carried out by \\{DNA\\} polymerase δ, the \\{PCNA\\} clamp, its loader RFC, and completed by \\{DNA\...
et al. The structural and biochemical characterization of human RNase H2 complex reveals the molecular basis for substrate recognition and Aicardi-Goutières syndrome defects. J. Biol. Chem. 286, 10540–10550 (2011). 6. Bubeck, D. et al. PCNA directs type 2 RNase H activity on DNA ...
These results show that RNase H2 is the preferred RNase H enzyme to target cDNA in yeast. Targeting of cDNA by RNase H2 does not require RNase H2 interaction with the DNA clamp proliferating cell nuclear antigen (PCNA). Moreover, yeast RNaseH2 orthologous mutants of two common RNase H2 ...
PCNA directs type 2 RNase H activity on DNA replication and repair substrates. Ribonuclease H2 is the major nuclear enzyme degrading cellular RNA/DNA hybrids in eukaryotes and the sole nuclease known to be able to hydrolyze ribonucleo... Doryen,Bubeck,AM Martin,... - 《Nucleic Acids Research...
PBS的工作pH为7.2~7.4,与人体血液等渗,主要由磷酸二氢钾(KH2PO4)、磷酸氢二钠(Na2HPO4)、氯化钠(NaCl)、氯化钾(KCl)组成。 本品为1×PBS,经RNase-free和除菌处理,可直接使用,适用于RNA杂交实验。本品不含钙离子和镁离子,不含酚红。 保存与运输方法 保存:室温保存,长期不用置于2-8℃保存,至少1年有效。
在原核生物中,RNase H2作为单体蛋白具有酶活性。在真核生物中,它是一种专性异源三聚体,由催化亚基A和结构亚基B和C组成。尽管亚基A与原核RNase H2紧密同源,但B和C亚基在原核生物中没有明显的同源性,即使在真核生物中在序列水平上也保守性很差。B亚基介导RNaseH2复合物和PCNA之间的蛋白质-蛋白质相互作用,其将...
PCNARNase H2RNase H2 of Saccharomyces cerevisiae consists of three essential subunits (Rnh201, Rnh202 and Rnh203) and plays a critical role in the removal of RNA incorporated in duplex DNA. In the present study, we purified individual subunits and heterodimeric subcomplexes to examine the ...
PCNAFEN1RNase H2Okazaki FragmentCryo-EMPCNA is a master coordinator of many DNA-metabolic events. During DNA replication, the maturation of Okazaki fragments involves at least four DNA enzymes, all of which contain PCNA-interacting motifs. However, the temporal relationship...
Table 2.Representative structures of RNase H2 proteins. OrganismPDB IDCommentsReference M. jannaschii1EKE—[107] A. fulgidus1I39, 1I3A—[108] 3P83complex with PCNA[109] T. maritima2ETJ—unpublished T. kodakarensis1X1Pamyloid β peptide fusion[110] ...