(5)电泳检测。 3. RIP(RNA Binding Protein Immunoprecipitation,RNA结合蛋白免疫沉淀) 该技术用来研究特定蛋白与全基因组RNA结合情况的技术。该技术主要借鉴了ChIP-seq的蛋白抗原抗体特异性结合的原理,利用特定的抗体将RNA-蛋白复合物进行富集,并用磁珠进行捕获,回收特定的RNA进行逆转录并测序。 其技术步骤如下: (1)...
J. M.; Florens, L.; Washburn, M. P.; Van Nostrand, E. L.; Pratt, G. A.; Schwinn, M. K.; Daniels, D. L.; Yeo, G. W., SONAR Discovers RNA-Binding Proteins from Analysis of Large-Scale Protein-Protein Interactomes. Molecular cell 2016, 64 (2)...
precisely mapping the binding sites of RNA-binding proteins, and also ensures that bona fide cross-linked [...] labome.com 这种在被测序的cDNA序列中的转变提供了一个巧妙的解决 准 确 绘 制 RNA 结 合 蛋白 的结 合位点方法,从而从噪声中获得了真实的交联的RNA序列。 labome.cn Antagomirs ...
从结果来看输入的蛋白和RNA是有结合的,再点击RNA-Binding Domains可以得到RNA可能结合在蛋白的哪一段序列,示例如下: 再点击RNA-Binding Motifs可以得到在RNA序列上发现的RNA结合序列的信息,示例如下: 依据matched_sequence的结果可以进行后续的RIP-seq以及RIP-qPCR等验证。爱基百客可以提供这两个技术服务,有相关需求的老...
2. Sodir, N. M. et al. Reversible Myc hypomorphism identifies a key Myc-dependency in early cancer evolution.Nat. Commun. 13, 6782 (2022). 3. Pereira, B., Billaud, M. & Almeida, R. RNA-binding proteins in cancer: old players and new actors.Trends Cancer3, 506–528 (2017)....
RBPs are proteins ubiquitously expressed across human tissues that recognize specific binding sequences and/or a secondary structures of RNA molecules controlling RNA metabolism either at the nuclear (transcription, splicing, capping, polyadenylation) or cytoplasmic (transport, localization, translation, degr...
总结来说，HydRA 不仅可以拓展人们对于 RNA 结合蛋白的认知范围，也能为相关疾病的治疗提供新启发和新可能。（来源：Molecular Cell）日前，相关论文以《HydRA：从蛋白质相互作用关联上下文和蛋白质序列预测 RNA 结合能力的深度学习模型》（HydRA: Deep-learning models for predicting RNA-binding capacity from protein ...
英文名称 Anti-Staufen/STAU1中文名称 双链RNA结合蛋白Staufen抗体浓度1mg/1ml 规格0.2ml/200μg抗体来源 Rabbit 克隆类型 polyclonal 交叉反应 Human, Mouse, Rat, Chicken, Cow, Horse 产品类型 一抗 别名Double stranded RNA binding protein Staufen homolog 1; Double stranded RNA binding protein Staufen homolo...
Following the realization that eukaryotic RNA-binding proteomes are substantially larger than anticipated, we must now understand their detailed composition and dynamics. Methods such as RNA interactome capture (RIC) have begun to address this need. Howe
Roquins are a family of highly conserved RNA-binding proteins that also contain a RING-type E3 ubiquitin ligase domain. They repress constitutive decay elements containing mRNAs and play a critical role in RNA homeostasis and immunological self-tolerance