Genetic variants (GVs), primarily including Single-Nucleotide Polymorphisms (SNPs) and Single-Nucleotide Variations (SNVs), are permanent changes in the nucleotides of DNA sequence that makes up a gene. The alternative alleles of a GV may confer different binding specificity to an RBP since each ...
Hybridization-based protocols require the presence of single-stranded RNA or DNA. Because the majority of respiratory viruses are single-stranded RNA, these viruses are believed to be suitable for detection with hybridization techniques. In this approach, a suitable oligonucleotide probe sharing a certai...
We computed the gene length as the mean length of all transcript isoforms included in the GENCODE basic gene set and with Transcript Support Level 1 in ensembl. Mean abundances per fraction were computed for the cytosol and membrane fractions, from which ratios were then computed compared with ...
然而,DP联合小干扰RNA(small interfering RNA,siRNA)靶向沉默的CCAT1抑制TNBC进展的机制研究尚未见报道。 Akt/糖原合成激酶-3β(glycogen synthase kinase-3β,GSK-3β)信号通路是细胞内重要的信号转导途径之一,在多种癌症的发生发展中起着重...
2 The basic properties of cotton lncRNAs and coding RNA 4570 中国农业科学 56 卷 2.2 亚洲棉短纤维起始期差异 lncRNA 的表达分析 为了鉴定出参与短纤维发育的候选 lncRNA,对 亚洲棉野生型和其突变体 4 个纤维发育时期(GA0149 vs GA0146 0 DPA,3 DPA,5 DPA 和 8 DPA)的 lncRNA 转录本表达量进行分析...
RNA viruses have the smallest genomes and the highest mutation rates, in some cases 100-fold higher than viruses with DNA genomes. High mutation rates are advantageous because the virus is able to quickly mutate gene products and evade host defense systems. Not surprisingly, an example of an RN...
(1)待测序的序列结构:使用第二代测序仪(illumina)进行测定的序列,无论是来自DNA-seq文库还是RNA-seq文库,自左向右划分为3个区:5’接头区(Adapter)、目标序列区(Insert)和3’接头区(Adapter)。 引自参考文章 (2)单端测序 vs 双端测序:单端测序(single-end)测序指的是从read1开始测,双端测序(pair-end)指的...
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XenosplitXenoSplit is a fast computational tool to detect the true origin of the graft RNA-Seq and DNA-Seq libraries prior to profiling of patient-derived xenografts (PDXs). single cell RNA-seq normalization Single cell RNA-seq a collection of single RNA-seq tools by Sean Davis ...