Ribosome-bound mRNA mapping (RIBOmap) enables three-dimensional in situ detection of spatially resolved protein synthesis in single cells. Lei Tang Article|07 August 2023 Unsupervised removal of systematic background noise from droplet-based single-cell experiments using CellBender ...
Bacteriophage T4 uses an enzyme known as ADP-ribosyltransferase ModB to modify the translational apparatus of bacteria it infects, not only by ADP-ribosylating proteins, but also by attaching entire RNA chains in a process known as RNAylation. ...
During the most recent rounds of directed evolution, which are reported here, the polymerases were required to synthesize the class I ligase. This requirement placed unprecedented selection pressure on the accuracy of synthesis because even a few mistakes would result in an inactive product. As a c...
The mRNA is an exact copy of the gene, in which all thymines (T) are replaced with uracils (U). In the second step, called translation, mRNA is used as a template for protein synthesis in a special cell machinery, the ribosome. Ribosome is a complex aggregate of special ribosomal RNA...
RNA synthesis is different from DNA synthesis. The most evident distinction is that in DNA replication, the newly lengthened DNA string contains...Become a member and unlock all Study Answers Start today. Try it now Create an account Ask a question Our experts can a...
RNA synthesis is quickly and significantly depressed when cells (both malignant and normal) are subjected to hyperthermia. To a lesser extent DNA and protein synthesis are also suppressed, although this may be secondary to the inhibition of RNA synthesis. These alterations in nucleic acid synthesis ...
(Fig.6). RNA editing, as a co/posttranscriptional modification, can change nucleotide sequences and have important effects on viral replication, protein synthesis, infection, and virulence. ADARs and APOBECs, in addition to deamination, are RNA-binding proteins that directly interact with endogenous...
The ion torrent sequencing platform is also based on the “sequencing by synthesis” method, which outperforms pyrosequencing with respect to sensitivity. SOLiD (Sequencing by Oligonucleotide Ligation and Detection) exhibits high accuracy, as each base is sequenced twice, but the read length is short...
Association of snR190 snoRNA chaperone with early pre-60S particles is regulated by the RNA helicase Dbp7 in yeast The molecular events underlying the assembly and maturation of the early pre-60S particles during eukaryotic ribosome synthesis are not well understood. Here, the authors combine yeast...
Replicative DNA polymerases cannot initiate DNA synthesis de novo and rely on dedicated RNA polymerases, primases, to generate a short primer. This primer is then extended by the DNA polymerase. In diverse archaeal species, the primase has long been known to have the ability to synthesize both ...