而如果Resolution越小,则cluster越少,会丢失很多信息,后续的亚群注释也难以进行,由此可见,resolution的设置在整个实验过程中是十分重要的~ 接下来小果给大家展示一下究竟是不是随着resolution的增高,cluster也会变多,在代码中我们将resolution从0.1到0.9进行循坏,通过dimplot来绘制降维的cluster图。 resolution_values<-seq...
Google search:RNA-seq library construction The impact of RNA sequence library construction protocols on transcriptomic profiling of leukemia Introduction to RNA-Seq for Researchers- YouTube - 整个过程讲得比较全面 Get more from your core: RNA-Seq techniques in action | Illumina Video- 13分 PCR-free...
Instrument LibraryLayout ## 1 SRX8718088 WT GSM4668511 HiSeq X Ten PAIRED ## 2 SRX8718089 WT GSM4668512 HiSeq X Ten PAIRED ## 3 SRX8718092 WT GSM4668515 HiSeq X Ten PAIRED ## 4 SRX8718093 WT GSM4668516 HiSeq X Ten PAIRED ## LibrarySelection LibrarySource Organism Platform ## 1 cDNA...
在这里,我们**于讨论常用的基于细菌和真核生物Illumina的RNA-seq协议的共性和差异(图3a),因为它们最接近发展成多物种RNA-seq方法。 图3:链特异性细菌、哺乳动物或双表达谱的常用方案的基本步骤。 a. 单种别RNA-seq。细菌RNA-seq协议(左)基于NEB Next Small RNA Library Prep Set 工作流(NEB Lab),哺乳动物协...
该方法结合DESeq2结果文件获取其他ID。使用org.Mm.eg.db包的mapIDs函数。 代码语言:javascript 代码运行次数:0 运行 AI代码解释 library(org.Mm.eg.db) # Add gene full name TAC_dge_result$description <- mapIds(x = org.Mm.eg.db, keys = row.names(TAC_dge_result), column = "GENENAME"...
前面RNA-seq分析:从软件安装到富集分析部分已经把转录组全部流程走完了一遍,这次利用RNA-seq(2)-2:下载数据中下载的肝癌数据进行分析,不在赘述细节,所以有看细节的还是请去这里。 这部分主要是代码和部分结果图,但进行了部分修正,比如KEGG 可视化部分,用了前clusterprofiler部分的结果,所以这部分不包括Gage包。 所以...
RNA seq名词解释 RNA-seq 名词解释 诺禾致源转录调控研究部 2014.03.21 基本概念 RNA-seq：基于二代测序技术，研究特定细胞在某一功能状态下所有RNA的功能，主要包括mRNA和非编码RNA。能够全面快速地获得某一物种特定组织或器官在某一状态下的几乎所有转录本序列信息，已广泛应用于基础研究、临床诊断和药物研发等...
Comparison of transformations for single-cell RNA-seq data This paper compares different transformation approaches for analysis of single-cell RNA-sequencing data and provides recommendations for method selection. Constantin Ahlmann-Eltze & Wolfgang Huber Article | 16 March 2023 Femtosecond laser micr...
RNA-seq library fragmentation and size selection strategies that influence interpretation and analysis.Malachi GriffithJason R. WalkerNicholas C. SpiesBenjamin J. AinscoughObi L. Griffith
SOLiD RNA-Seq library preparation and sequencing Similar to the Illumina platform, a workgroup was also formed with representatives from the three official SOLiD sequencing sites to reach consensus on an SEQC-specific sequencing SOP (standard operating procedure) based on the low input protocol of ...