Accurate identification and effective removal of unwanted variation is essential to derive meaningful biological results from large and complex RNA-seq studies. Technical replicates together with negative and positive control genes are key tools for carrying out this task. We show how to proceed when ...
另外RNA-Seq建库有两种方法：一种是题主你说的这种，针对mRNA进行片段化，比如碱处理法、酶（RNaseIII）处理法片段化。然后反转录、测序，这样获得的reads一般是针对基因body区的。另外一种可以先转录成cDNA得到长片段，然后再进行类似DNA文库构建方法，比如酶切法、超声打断，但是这样的建库方式对转录本的3'末端偏好...
另外RNA-Seq建库有两种方法：一种是题主你说的这种，针对mRNA进行片段化，比如碱处理法、酶（RNaseIII...
产品名称 RNA Seq 文库构建试剂盒 有效成分含量 100% 是否进口 是 用途范围 科研 英文名称 RNA Seq Library Prep Kit 输入纯化RNA量 从3 ng到100 ng 产地 美国 供货商 北京百奥创新科技有限公司 代理 全国总代 售卖区域 全国 价格 详询 可售卖地 北京;天津;河北;山西;内蒙古;辽宁;吉林;黑龙江;上海...
It is the ideal solution for total RNA-Seq analysis on degraded and FFPE samples. New! Adjustable library size to perfectly fit your application: CORALL RNA-Seq V2 allows generation of longer libraries (~ 550 bp) for demanding applications such as alternative splicing, isoform analysis, and ...
library preparationnext-generation sequencingRNA-seqDNA-seqChIP-seqRIP-seqHigh-throughput sequencing, also known as next-generation sequencing (NGS), has ... SR Head,HK Komori,SA Lamere,... - 《Biotechniques》 被引量: 182发表: 2014年 Transcriptome Sequencing: RNA-Seq Library constructionMessenger...
可以深挖一下,做人RNA-seq数据分析的时候,没有考虑到人种的问题,能不能搞个每个人种的附加转录本,本质就是hg19和hg38的补充,以及注释的完善。 人种特异的转录本,需要高效的算法,按人种合并到一起,这些转录本是否有人种特异的表征,肤色? 添加这些novel的转录本后mapping rate有多少提升?能否最终把unmapped rate降到...
effector gene library are co-delivered to protoplasts such that a subpopulation of cells expressing theAvrgene undergoes cell death, resulting in the depletion ofAvrgene transcripts in the living cell population. Library-specific RNA-seq and differential gene expression analysis is then used to ...
Zymo-Seq RiboFree Total RNA Library Kit价格:￥14157/盒产品详情:南京沃博生物科技有限公司是一家专注于生命科学和生物技术领域,致力于为客户提供一系列高质量和有绝对价格竞争力的产品,包括分子... 纯度:详见说明% 包装规格:12 Preps 用途:科研使用
In principle, RNA-Seq can measure and quantify known genes as well as novel genes,isoformsand non-codingRNAs(ncRNAs). By employing this technology, it was found that over 90% of the human genes undergo alternative splicing to create at least one isoform from one gene (Wanget al.,2008). ...