RNA dependent RNA Polymerase (RdRp) is an enzyme that is responsible for the replication of RNA from an RNA template. From: Bioorganic Chemistry, 2020 About this pageAdd to MendeleySet alert Also in subject areas: Medicine and Dentistry Nursing and Health Professions Agricultural and Bio...
we find that the translation inhibitor causes genome-wide premature stalling of RNA polymerase (RNAP) in a loci-dependent manner. Further analysis shows that the stalling is caused by the disruption of transcription-translation coupling. Anti-intuitively, the stalled RNAPs subsequently induce lesions ...
(total length of 31 kb). The fourth component of rRNA is5S RNAwhich is transcribed byRNA polymerase III. The 5S rRNA gene is not normally linked to that of other rRNAs. Together withribosomal proteins, the18S rRNAconstitutes the small 40S ribosomal subunit; 5S, 5.8S, and 28S rRNAs ...
RNA polymerase II regulates topoisomerase 1 activity to favor efficient transcription. Cell 2016; 165: 357–371. 59 Huang CH, Lujambio A, Zuber J, Tschaharganeh DF, Doran MG, Evans MJ et al. CDK9-mediated transcription elongation is required for MYC addiction in hepa- tocellular carcinoma....
Glutaminase (GLS) is directly related to cell growth and tumor progression, making it a target for cancer treatment. The RNA-binding protein HuR (encoded by the ELAVL1 gene) influences mRNA stability and alternative splicing. Overexpression of ELAVL1 is
The present invention provides engineered RNA polymerase variants and compositions comprising these variants. The present invention further provides engineered T7 RNA polymerase var
cDNA was synthesized from 2 μg total RNA using 1 μg random hexamers by Superscript III Reverse Transcriptase (Invitrogen). PCR fragments for sequencing were obtained using the Phusion High Fidelity DNA Polymerase kit (NEB M0530C) on the synthesized cDNA, followed by purification of the PCR ...
Provides a rapid and efficient way to clone double-stranded oligonucleotide (ds oligo) duplexes encoding a desired shRNA target sequence into an entry vector containing an RNA Polymerase III (Pol III)-driven expression cassette (i.e. H1/TO RNAi cassette) ...
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Despite the absence of known biological examples of RNA enzymes with RNA-dependent RNA polymerase activity (RNA replicase), substantial advances have been achieved in laboratories through directed evolution methods [10,11,12,13,14]. In particular, the most advanced examples of laboratory-created ...