StephenBuratowski, inCurrent Opinion in Cell Biology, 2000 RNApolymerasecan be envisaged as a roughly ‘crab claw’ or ‘clam’ shaped molecule, with the bottom half consisting mainly of the largest subunit β′ and the upper half containing most of the second largestsubunit β. Several channels...
RNA PolymeraseCellular CompartmentProduct of Transcriptionα-Amanitin Sensitivity I Nucleolus All rRNAs except 5S rRNA Insensitive II Nucleus All protein-coding nuclear pre-mRNAs Extremely sensitive III Nucleus 5S rRNA, tRNAs, and small nuclear RNAs Moderately sensitiveRNA...
Related to RNA-dependent RNA polymerase:replicase pol·y·mer·ase (pə-lĭm′ə-rās′, -rāz′, pŏl′ə-mə-) n. Any of various enzymes, such as DNA polymerase, RNA polymerase, or reverse transcriptase, that catalyze the formation of polynucleotides of DNA or RNA using an ...
Qiaozhu Su, in Advances in Protein Chemistry and Structural Biology, 2022 3.5 RNA transcription NSP12-16 comprise the core functions of viral RNA synthesis, proofreading, and modification. The RNA-dependent RNA polymerase (RdRP) residing in NSP12 is the centrepiece of coronavirus RTC and...
RNA polymerase I (Pol I) specifically synthesizes ribosomal RNA. Pol I upregulation is linked to cancer, while mutations in the Pol I machinery lead to developmental disorders. Here we report the cryo-EM structure of elongating human Pol I at 2.7 Å resolution. In the exit tunnel, we obs...
we find that the translation inhibitor causes genome-wide premature stalling of RNA polymerase (RNAP) in a loci-dependent manner. Further analysis shows that the stalling is caused by the disruption of transcription-translation coupling. Anti-intuitively, the stalled RNAPs subsequently induce lesions ...
Learn about the process of transcription inside a cell with a focus on the structure and function of the RNA polymerase ii in the process.
“ir” refers to a model in which both splice sites can be used or not used, similar to the “intron retention” event in classical definition. We used the SUVA pipeline to detect AS events from short read RNA-seq data based on the five different splicing patterns described above. In ...
Hepatitis C virus utilizes flavin adenine dinucleotide as a non-canonical initiating nucleotide for the viral RNA polymerase, resulting in 5′ capping of viral RNA, which provides protection against the host innate immune response. Anna V. Sherwood ...
Elongin is a heterotrimeric elongation factor for RNA polymerase (Pol) II transcription that is conserved among metazoa. Here, we report three cryo-EM structures of human Elongin bound to transcribing Pol II. The structures show that Elongin subunit ELOA