RNA Polymerase is the enzyme responsible for reading a DNA sequence and transcribing it into RNA. It functions by melting the double-stranded DNA template and moving along the DNA while synthesizing RNA in a template-directed manner. AI generated definition based on: Single Molecule Biology, 2009...
RNA polymerase II is the enzyme responsible for the synthesis of mRNA and many non-coding RNAs. POLR2A encodes RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II. The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence ...
For what purpose is the polymerase chain reaction (PCR) used? Does a retrovirus carry RNA polymerase? If RNA polymerase is responsible for separating DNA strands during transcription, then what does Helicase do? How does DNA polymerase interact with DNA? What happens if an amino acid sequence ha...
RNA polymerase I (Pol I) specifically synthesizes ribosomal RNA. Pol I upregulation is linked to cancer, while mutations in the Pol I machinery lead to developmental disorders. Here we report the cryo-EM structure of elongating human Pol I at 2.7 Å resolution. In the exit tunnel, we obs...
(a) What is RNA polymerase? (b) What is its function? What is responsible for the replication of mRNA? What are the role of DNA polymerase and RNA polymerase? What is polymerase chain reaction (PCR) and what impact does it have in DNA and RNA?
ChIP was performed on sheared chromatin from 1 million HeLaS3 cells using 1 µg RNA Polymerase II S2p monoclonal antibody (Product #MA5-23510). The IP'd DNA was subsequently analyzed on a Genome Analyzer. The 36 bp tags were aligned to the human genome using the ELAND algori...
RNA polymerase III (Pol III) transcribes noncoding RNA, including transfer RNA (tRNA), and is commonly targeted during cancer and viral infection. We find that Herpes Simplex Virus-1 (HSV-1) stimulates tRNA expression 10-fold. Perturbation of host tRNA synthesis requires nuclear viral entry, ...
1976). R-loops were originally considered an extension of the RNA:DNA hybrid found within the RNA polymerase II transcription bubble (Westover et al. 2004), but it appears more likely that they result from the fold-back of nascent RNA as it exits the polymerase, known as an RNA thread ...
The RNA Pol II stalk-to-stalk dimer could also be involved in gene looping and polymerase recycling, possibly mediating contact between gene promoter and terminator. The dimerization surface could also be involved in other transcription processes, including initiation and capping, complicating the interp...
For experiment of half-life, cells were grown to ~50% confluency before RNA polymerase activity was blocked by 10μg ml−1actinomycin D (sigma) in DMSO. Transcription inhibition of K562 was carried out for 30 hours, and cells were harvested at time 0 h, 0.5 h, 2 h, 4 h, 8 h, ...