Macromolecular micromovements: how RNA polymerase translocates. Curr. Opin. Struct. Biol. 2009; 19 :701–707.Svetlov, V., and Nudler, E. (2009) Macromolecular micromovements: how RNA polymerase translocates. Curr Opin Struct Biol 19, 701-707...
How does gel electrophoresis separates DNA fragments? How is the DNA structure arranged as a double helix? How are DNA and mRNA alike? What is the importance of DNA sequencing? How does DNA electrophoresis work? What helps RNA polymerase recognize the start of a gene?
How does RNA polymerase choose its DNA strand to transcribe? How do viruses get their DNA or RNA? How are DNA, RNA, and proteins related in the cell? Describe how the DNA code is read through the processes of Transcription and Translation. ...
To make mRNA, RNA polymerase: Binds to the DNA strand at a specific sequence of the gene called a promoter Unwinds and unlinks the two strands of DNA Uses one of the DNA strands as a guide or template Matches new nucleotides with their complements on the DNA strand (G with C, A with...
The RNA protected by these dimers was found to be derived from two distinct regions of the genomic RNA, one from the RNA polymerase gene around the leaky termination codon and the other corresponding to the coat protein gene (Wei et al., 1990). While these studies did not arise directly ...
These include covalent modifications of chromatin, structural changes and modulation of RNA polymerase II activity. Mechanisms of silencing and activation antagonize each other. During the cell cycle, replication and mitosis are the stages at which epigenetic memory is most likely to be erased. Each ...
In the absence of lactose, the LacI protein binds to the promoter of the lac operon, preventing RNA polymerase (RNAP) from binding. This means that the rest of the lac operon (lacZ, lacY, and lacA) can’t be expressed. Because lactose is not metabolized as easily as glucose, the ...
How Does the CRISPR-Cas System Work? CRISPR is the coolest technology in a biologist’s toolbox because of its unique blend of being extremely powerful yet relatively simple to use. The technique relies on two basic components: a Cas nuclease that cuts the DNA and a guide RNA that tells ...
Directed evolution of novel polymerase activities: mutation of a DNA polymerase into an efficient RNA polymerase. Proc. Natl Acad. Sci. USA 99, 6597–6602 (2002). Article CAS PubMed PubMed Central Google Scholar Chen, T. et al. Evolution of thermophilic DNA polymerases for the recognition ...
Nearly 40 years ago scientistsfoundthat they could mimic transcription and produce synthetic mRNA without a cell. The process, known as in-vitro transcription, can generate many mRNA molecules from a strand of DNA in a test tube. This requires an enzyme (called RNA polymerase) and nucleotides ...