mRNA vaccine has emerged as a potentially useful alternative to DNA vaccine for using in the prevention of infectious illnesses and the treatment of cancer
Nases (RNase A and V1, respectively)25,29. This led to the proposal that the VA-I apical region exists as two structurally different, functionally distinct conformers25,29. However, it is perplexing how a single RNA region is both extraordinarily stable and yet fluid enough to diverge into...
DSN operates efficiently at higher temperatures and requires at least ten perfectly complementary base pairs to cut, thus it has better mismatch discrimination than RNase H, especially given the higher Tm of RNA:DNA compared to DNA:DNA hybrids. That PDD is performed downstream of adapter ligation ...
It is advised to validate more than one HKG for the normalization of qPCR data to avoid misleading results [28,29]. By validating housekeeping genes, we can ensure the reliability and reproducibility of qPCR experiments and minimize the risk of misinterpreting gene expression data. In the ...
). The conversion of single-stranded DNA into double-stranded DNA by DNA polymerase activity can be detected as an increase in total DNA length, at stretching forces below ∼6pN, or as a decrease when the force is higher than this “crossover” force (...
The AllPrep DNA/RNA Micro and Mini Kits can be automated on theQIAcube Connect. The AllPrep DNA/RNA 96 Kit is well suited for high-throughput purification of genomic DNA and total RNA from up to 96 samples. Performance Small samples of cultured cells and easy-to-lyse tissues can be proc...
Interestingly, we found that Pangolin’s performance is substantially better for variants near splice sites (AUPRC of 0.75, distance of 0-9 bases) than for farther variants (AUPRC <0.35, distance >9 bases), which may be attributable to the relative rarity of distant splice-disrupting variants...
theme explores the many different ways in which Z-formation alters the readout of genomic information over relatively short time-periods. The change is not as fast as observed with post-translational modifications, such as phosphorylation or ubiquitination. The time scale is better suited to feed...
These results show thatCRIPSRLwaCas13a can be reprogrammed with guide RNAs to effectively knockdown or bind transcripts in mammalian cells. LwaCas13a knockdown is comparable to RNAi knockdown efficiency, but with substantially reduced off-targets, making it potentially well-suited for therapeutic applica...
Pol I is optimally active at pH 7.4 in potassium phosphate buffer (50 mM) with either native DNA or poly(dAT) as template-primers (3). At pH 7.0 and 7.8, the polymerase activity is 70% of the optimal value. At pH 7.0, the polymerase activity is approximately twofold higher ...