2024年5月,杜克大学Fan Yuan教授团队在Advanced Drug Delivery Reviews(一区,IF=15.2)上发表题目为“A comprehensive comparison of DNA and RNA vaccines”的综述文章,系统比较了目前文献中已知的mRNA和DNA疫苗在开发时间表、财务成本、运输、疗效、安全性和产品监管批准诸多方面的优缺点,极大的方便了研究人员快速了解...
该参数能更好的反应测序数据的有效利用率,减少假阴性(有较高的目标区域覆盖率,但是具有较低的覆盖深度) 2、文章提出了双链RNA和单链DNA混合捕获的设想,因为不同类型探针的特征是不一致的,能更有效的降低 AT/GC dropout 5 参考文献 [1] Zhou J , Zhang M , Li X , et al. Performance comparison of fou...
2024年5月,杜克大学Fan Yuan教授团队在Advanced Drug Delivery Reviews(一区,IF=15.2)上发表题目为“A comprehensive comparison of DNA and RNA vaccines”的综述文章,系统比较了目前文献中已知的mRNA和DNA疫苗在开发时间表、财务成本、运输、疗效、安全性和产品...
DNA and RNA both carry genetic information, but there are quite a few differences between them. For example, DNA's double-stranded structure provides stability and protection for genetic information, while RNA's single-stranded nature makes it more chemically reactive and prone to being broken down...
表2. NSCLC队列中DNA-NGS检测到的26例“基因-基因间区域”融合模式病例及恒特基因RNA-NGS检测的结果比较 该研究结果:1. 进一步证明了DNA-NGS检测到基因组上基因融合拼接断点信息无法有效预测转录组上的真实融合拼接模式,在DNA层面上表现为复杂或罕见的融合,相当比例的在RNA 层面上实质为非常经典的融合;2. 并发现了...
Pearsons product moment correlation coefficient (r) for comparison between RINeand DIN for the samples stored at − 80 °C was − 0.33. For the samples stored at VPLN, r was − 0.19. The RINe- and DIN-values, including the 28 s/18 s Ratio and the RNA concentration of each sampl...
基于RNA检测融合基因,RT-PCR与NGS检测敏感度相当。相比NGS,RT-PCR在检测成功率、检测时间和技术普及度均优势显著。 参考文献 [1] Seo Jeong-Sun,Ju Young Seok,Lee Won-Chul et al. The transcriptional landscape and mutational profile of lung adenocarcinoma.[J] .Genome Res, 2012, 22: 2109-19. ...
近日,美国Ambry Genetics公司在npj Genomic Medicine杂志上发布了一项大规模队列研究的结果,文章题为“Mutational and splicing landscape in a cohort of 43,000 patients tested for hereditary cancer”。研究团队通过将DNA和RNA测序配对来解决剪接变体识别和解释的难题,并描述了由43,524名接受遗传性癌症易感基因检测...
融合基因DNA和RNA水平上的结构特征决定了融合基因检测,RNA水平远比DNA水平更敏感,更准确。 基于RNA检测融合基因,RT-PCR与NGS检测敏感度相当。相比NGS,RT-PCR在检测成功率、检测时间和技术普及度均优势显著。 参考文献 [1] Seo Jeong-Sun,Ju Young Seok,Lee Won-Chul et al. The transcriptional landscape and mu...
摘要: The analytical procedure for the quantification of DNA and RNA described is the quantitative visual comparison of an unknown sample with known standards. This is quick and easy to do, suitable for amounts of sample as small as 10 ng. Keywords: DNA; RNA; rapid quantification关键词:...