This differs from gene knockdown by antisense methods, in which silencing is determined by the local mRNA conformation.doi:10.1016/j.csda.2004.06.017Simon FrantzNature Reviews Drug DiscoveryFrantz, S. "RNA Interference: Eight Steps to silence" Nature Reviews Drug Dscovery 2004, 3 (3), 204...
RNA interference (RNAi) is a natural cellular process that regulates gene expression by a highly precise mechanism of sequence-directed gene silencing at the stage of translation by degrading specific messenger RNAs or blocking translation. In recent years, the use of RNAi for therapeutic applications...
Micro RNA (miRNA) is made by processing a longer precursor that folds into a stem loop. Processing occurs in two steps using the nucleases Drosha and Dicer. After binding to miRISC and strand separation, one strand of the miRNA binds to the target mRNA and prevents translation. ...
2b), or their RNA interference (RNAi) depletion, robustly increased CPL-1* protein levels, as seen in sel-11(hh17) (Fig. 1d,e and Extended Data Fig. 2c,d). CPL-1* co-fractionated with SEL-1 in ER-derived microsomes of rde-1(ne219) worms (Fig. 1f), indicating that the CPL-...
[2]Palli, S. R. (2014). RNA interference in Colorado potato beetle: steps toward development of dsRNA as a commercial insecticide. Curr. Opin. Insect Sci.6, 1–8. doi: 10.1016/j.cois.2014.09.011 [3]Kolliopoulou, A., and Swevers, L. (2014). Recent progress in RNAi research in ...
Single-stranded, DNA-damage-associated small RNAs generated by a BRCA1–RNA-interference complex promote PALB2–RAD52-mediated DNA repair at transcriptional termination pause sites that contain R-loops and are rich in single-stranded DNA breaks in both quiescent and proliferating cells. ...
Micro RNA is found in worms, insects, mammals, and plants—that is, the same organisms that display RNA interference. The first miRNAs were found in Caenorhabditis elegans where they were called small temporal RNA (stRNA) because they regulate the timing of worm development during the conversion...
RNA-based gene therapies are a candidate, which includes antisense RNA, RNA decoy, ribozyme, RNA interference (RNAi), and Cas9/single guide RNA (sgRNA) technologies. The last three decades have witnessed tremendous progress in the development of these RNA-related approaches for HIV. Their prowess...
RNA interference (RNAi) is an evolutionarily conserved phenomenon of double-stranded (ds)RNA-mediated mRNA degradation that leads to the posttranscriptional silencing of the corresponding gene. First reports on RNAi emerged in 1998, and since then, it has become one of the most fascinating fields ...
In plants, RNA silencing (RNA interference) is an efficient antiviral system, and therefore successful virus infection requires suppression of silencing. Although many viral silencing suppressors have been identified, the molecular basis of silencing suppression is poorly understood. It is proposed that ...