Regulation at the RNA Level 2Basic Principles of RNA Interference (RNAi) RNAinterference (RNAi)is a mechanism for gene silencing that is induced by double-stranded RNA (dsRNA). It is sequence specific and invol
Howard KA. RNA Interference from Biology to Therapeutics . New York: Springer; (2012).Howard KA. RNA Interference from Biology to Therapeutics. New York: Springer (2012).Pittella, F. & Kataoka, K. in RNA Interference from Biology to Therapeutics (ed. Howard, K. A.), 161-184 (Springer ...
RNA interference, or RNAi, is a widely used technique in which double stranded RNA is introduced to an organism, resulting in targeted gene silencing. The Nobel winning discovery of RNA interference allowed researchers to silence any C. elegans gene in order to determine its function. We can in...
VI. The Future of RNA Interference in Biology and Medicine Even at this early stage of understanding the molecular mechanisms of RNAi and in the development of methods for the use of RNAi technology for selective gene silencing, it is clear that RNAi will be a widely used tool for establishin...
Single-stranded, DNA-damage-associated small RNAs generated by a BRCA1–RNA-interference complex promote PALB2–RAD52-mediated DNA repair at transcriptional termination pause sites that contain R-loops and are rich in single-stranded DNA breaks in both quiescent and proliferating cells. Elodie Hatchi...
Mello, the two 2006 Nobel laureates "for their discovery of RNA interference - gene silencing by double-stranded RNA" (http://nobelprize.org/nobel_prizes/medicine/laureates/2006/). Well, it is impressive how both the Nobel laureates clearly anticipate the immense possibility the phenomenon they ...
Double-stranded RNA-mediated interference (RNAi) is a simpleand rapid method of silencing gene expression in a range oforganisms. The silencing of a gene is a consequence of degradationof RNA into short RNAs that activate ribonucleases to targethomologous mRNA. The resulting phenotypes either are ...
A. The RNAi revolution. Nature 430, 161–164 (2004) Article ADS CAS PubMed Google Scholar Dykxhoorn, D. M. & Lieberman, J. The silent revolution: RNA interference as basic biology, research tool, and therapeutic. Annu. Rev. Med. 56, 401–423 (2005) Article CAS PubMed Google ...
The observed fast turnover of circRNAs upon poly I:C treatment was not due to the transcriptional level interference, but specific to poly I:C and EMCV. The catalytic activity of RNase L was essential for circRNA degradation. It has been known for long time that pathogenic dsRNA binds to ...
在线虫中,已经筛选到了RNA转运的相关基因——SID(systemic RNA interference defective, 系统性RNA干扰缺陷),SID有多种、下属多种功能蛋白(↓)。另外还发现了一些与RNA跨细胞转移相关的基因,在此就不列举了,具体可以参考ref[1]。 SID-1选择性转入dsRNA、不区分小RNA的具体长度,但与RNA转出细胞无关、在外界诱导的...