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2. Strecker, J. et al. RNA-guided DNA insertion with CRISPR-associated transposases.Science365, 48–53 (2019)
2. Strecker, J. et al. RNA-guided DNA insertion with CRISPR-associated transposases.Science365, 48–53 (2019)
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近期,来自立陶宛维尔纽斯大学的Tautvydas Karvelis和Virginijus Siksnys等人发现来自耐辐射球菌ISDra2的TnpB是一个RNA导向的核酸酶,相关文章发表在Nature:Transposon-associated TnpB is a programmable RNA-guided DNA endonuclease。 作者首先发现在大肠杆菌中表达tnpB需要与tnpA一起,说明可能需要额外的转座子元素稳定它...
IS200/IS605 和 IS607 家族的插入序列 (insertion sequences, ISs)是最简单的可移动遗传元素之一,仅包含其转座和调节所需的基因。2021年9月9日,张锋团队在Science杂志上发表了一篇题为The widespread IS200/605 transposon family encodes diverse programmable RNA-guided endonucleases的文章(详见BioArt报道:Science ...
TAM sequence requirements of TnpB proteins, reflecting evolutionary relationships between Cas12 nucleases and MGEs of the IS200/IS605/IS607 families. RNA-guided DNA insertion by CRISPR-associated Tn7-like transposons provides another example of the interplay between MGEs and CRISPR–Cas systems31,...
最终依赖 RNA 连 接酶将 mRNA 连接起来,这一系列酶促反应完成编 辑过程.这种编辑形式首先在锥虫线粒体中发现[2], 其细胞色素 C 氧化酶亚基Ⅱ(Cox Ⅱ)转录本与基 因序列不匹配,存在 4 个非 DNA 编码的尿嘧啶,后来在变形菌(Proteusbacillus vulgaris) 和副粘病毒 (Paramyxoviridae)基因中也分别发现了 C ...
transposongenome editingCASTcryo-EMThe type V-K CRISPR-Cas system, featured by Cas12k effector with a naturally inactivated RuvC domain and associated with Tn7-like transposon for RNA-guided DNA transposition, is a promising tool for precise DNA insertion. To reveal the mechanism underlying target...