During elongation, the RNA polymerases must be processive, and once they begin an RNA molecule they must finish it without dissociating from its template. To guarantee this fundamental necessity, the transcriptional machinery contains all the factors that permit the polymerase to overcome the physical ...
complex (CCR4–NOT complex)37,38to the m6A-containing mRNA via a direct interaction with the N-terminus of YTHDF2 and the SH domain of CNOT1, a component of the CCR4–NOT complex33. The resulting deadenylated m6A-containing mRNA would be more vulnerable to 3′–5′ exoribonucleolytic c...
tRNA has three large single-stranded loops. Theanticodonloopcontains seven bases. The loop containing bases 14–21 is called theD loop(or the DHU loop); itis not constant in size in different tRNA molecules. The loop containing bases 54–60 almost always contains the sequence TψC and is ...
The NF1 gene contains hotspots for L1 endonuclease-dependent de novo insertion. PLoS Genet. 7, e1002371 (2011). CAS PubMed PubMed Central Google Scholar Burns, K. H. Our conflict with transposable elements and its implications for human disease. Annu. Rev. Pathol. 15, 51–70 (2020)....
To validate our rG4-seq, we mapped RT stops on the mRNA of SUPPRESSOR OF MAX2 1-LIKE5 (SMXL5) which contains a G-rich region with RG4 folding potential identified recently [9]. We found a strong RT stalling at the 3′end of this G-rich region where the coverage dropped in the ...
The RNA sequence in Figure 1 is an example of a specific one-dimensional structure. Figure 1. Primary RNA Structure. When RNA molecules fold onto themselves, some nucleotides form base pairs through the creation of hydrogen bonds between complementary bases, where A pairs with U, U pairs ...
In addition to single-copy RNA transcripts, the human genome contains many high-copy regions that are expressed as functional RNAs but present a substantial challenge to standard short read mapping strategies. These include RNAs such as the large and small ribosomal RNA (rRNA), 7SK snRNA, and ...
AGO2 belongs to the highly conserved Argonaute family of proteins. Argonaute proteins are ∼100 kDa highly basic proteins that contain two common domains, namely the PIWI and PAZ domains.[7] The PIWI domain is crucial for the interaction with Dicer and contains the nuclease activity responsible...
The annotation used to compute the accuracy of transcriptome assemblies and to create the human short-read simulated data and the annotation-guided assemblies contains all full-length protein and long non-coding RNA transcripts from RefSeq, release GRCh38.p8. The A. thaliana RNA-seq reads were ...
Each 3D spectrum contains two 2D spectra, corresponding to the \({N}^{\alpha }\) and \({N}^{\beta }\) components of the magnetization, respectively. Analysis of 1HN CEST data All NMR spectra were processed and analyzed using NMRPipe, with peak intensities extracted with the autofit ...