核糖核酸(RNA)是一种重要的生物大分子,存在于所有生物细胞中。 【1】It isprincipallyinvolved in the synthesis of proteins, carrying the messenger instructions from DNA, which itself contains the genetic instructions required for the development and maintenan...
assuring the error rate as low as 10-5. The extra “proofreading” exonuclease activity of DNA polymerase improves the overall accuracy by recognizing and removing the new-coming mismatched bases (down to 10-7 error rate). The following repair system...
3.Order anti-sense DNA probes with BiotinTEG at 3-prime end. 4.Label probes according to their positions along the RNA. Separate them into two pools so that the "even" pool contains all probes numbering 2, 4, 6, etc. and the "odd" pool contains...
Bacterial RNApolymeraseis the smallest and simplest of the cellularRNA polymerases. The molecular weight of the RNA polymerase is about 470,000Da. It has a multisubunit structure which contains five different types of subunits, including α2, ω, β, β′, and σ. In other words, two molecu...
In addition, 82% of gene fusions were associated with structural variants, including 75 of a new class, termed ‘bridged’ fusions, in which a third genomic location bridges two genes. We observed transcriptomic alteration signatures that differ between cancer types and have associations with ...
Current single-cell RNA sequencing (scRNA-seq) methods with high cellular throughputs sacrifice full-transcript coverage and often sensitivity. Here we describe Smart-seq3xpress, which miniaturizes and streamlines the Smart-seq3 protocol to substantially
Because the nucleo-bases are exposed in a single-stranded ASO, these oligonucleotides are amphipathic. Phosphorothioate (PS) substitution of the phosphate groups reduces the hydrophilicity, stabilizes the ASOs to nuclease degradation, and enhances protein binding, which is critical to cell uptake and in...
Brown CJ, Hendrich BD, Rupert JL, Lafreniere RG, Xing Y, Lawrence J, Willard HF (1992) The human XIST gene: analysis of a 17 kb inactive X-specific RNA that contains conserved repeats and is highly localized within the nucleus. Cell 71:527–542. https://doi.org/10.1016/0092-8674(92...
ADARs deaminate adenosine in dsRNA or the A-form helix (DNA: RNA hybrid) [46,47,48]. The introduction of non-canonical base pairs into the dsRNA structure increases complexity, which actually affects the efficiency of adenosine editing (Fig.2). The two bases next to the edited adenosine in...
To validate our rG4-seq, we mapped RT stops on the mRNA of SUPPRESSOR OF MAX2 1-LIKE5 (SMXL5) which contains a G-rich region with RG4 folding potential identified recently [9]. We found a strong RT stalling at the 3′end of this G-rich region where the coverage dropped in the ...