Coding region determinant-binding protein (CRD-BP) is an RNA binding protein that regulates expression of cancer-associated genes such as c-myc. Thus, it is not surprising that CRD-BP is deregulated in several human cancers, including breast cancer. However, the mechanisms through which CRD-BP...
[1] Lunde, B. M.; Moore, C.; Varani, G., RNA-binding proteins: modular design for efficient function. Nature reviews. Molecular cell biology 2007,8 (6), 479-90. [2] Wheeler, E. C.; Van Nostrand, E. L.; Yeo, G. W., Advances and challenges in the detection of transcriptome-...
a function recapitulated with YFP-3′UTR reporters. DAZL binds to mRNAs whose translation is both repressed and activated during maturation. Injection of recombinant DAZL protein in DAZL-depleted oocytes rescues the translation and maturation to MII. Mutagenesis of putative DAZL-binding sites in these...
RNA-binding proteins (RBPs) play an essential role in regulating the function of RNAs in a cellular context, but our ability to control RBP activity in time and space is limited. Here, we describe the engineering of LicV, a photoswitchable RBP that binds to a specific RNA sequence in resp...
However, as the three sRNAs characterized in Caulobacter either function in the absence of the protein (ChvR) or the requirement of the chaperone has not been addressed (GsrN, CrfA), Hfq-dependent regulation by an sRNA still awaits experimental confirmation in this species. Sign in to download...
The RGG domains of MRE11 as well as the RNA-processing factor hnRNPUL1 mediate recruitment to sites of DNA damage (54, 75, 77), suggesting that this motif may function generally as an atypical PAR binding domain. The fact that FUS targeting to DNA damage was not fully supported by RGG...
总的来说,课题组构建了一个可编程的光控LicV,可实现RNA效应子的时空控制。 相关文章发表在Nature Biotechnology:Optogenetic control of RNA function and metabolism using engineered light-switchable RNA-binding proteins。 (王利钰 摘译)
RIP技术(RNA Binding Protein Immunoprecipitation,RNA结合蛋白免疫沉淀)是研究细胞内RNA与蛋白结合情况的技术。运用针对目标蛋白的抗体把相应的RNA-蛋白复合物沉淀下来,然后经过分离纯化就可以对结合在复合物上的RNA进行分析;即用抗体或表位标记物捕获细胞核内或细胞质中内源性的RNA结合蛋白,防止非特异性的RNA的结合(RIP...
许多研究已证实来源于同源结构域相互作用蛋白激酶3(homeodomain-interacting protein kinases 3, HIPK3)的circHIPK3表达的改变可影响多种恶性肿瘤细胞的侵袭和转移行为[43-44]。Zhang等[45]研究表明,子痫前期患者胎盘中circHIPK3下调,沉默circHIPK...
RBPs can also directly modulate protein translation, often through binding at the 5’UTR of RNA transcripts. In so doing, RBPs recruit translation initiation factors, adjust the accessibility of the RNA to ribosomes, create ribonucleoprotein structures conducive for cap-independent and internal ribosom...