FUS is normally localised to the nucleus and is involved in the regulation of various RNA processing events including alternative splicing, microRNA biogenesis, mRNA stability, transcription, translation and transport, and is also a well-known component of cellular stress granules [9]. It is also ...
For reverse transcription, any commercially available reverse transcription enzyme with random primers or oligo dT primers may be used. Note: To make a 1% Input, prepare a 1:10 dilution of the 10% Input samples from Page 9 and 10 in RNase free water. DNase treatment using DNase I, ...
At each step, we compared the alignment statistics, the number of differentially expressed genes (RNA-seq) or identified transcription factor binding peaks (ChIP-seq), and the results of Gene Ontology, KEGG and Reactome pathway enrichment analyses (RNA-seq) and motif enrichment analyses (ChIP-seq...